T. Schiffner

Diverse competitor B cell responses to a germline-targeting priming immunogen in human Ig loci transgenic mice

The paper analyzes B cell responses to a germline-targeting immunogen in human Ig loci transgenic mice, finding diverse non-VRC01-class B cells and competitor B cells recognizing epitopes near the VRC01 epitope.

Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

This paper shows that displaying GM3g glycans on proteins using specific chemical linkers elicits strong, germline-encoded B cell responses that do not undergo affinity maturation.

DeGlyPHER: an ultrasensitive method for analysis of viral spike N-glycoforms

DeGlyPHER is an ultrasensitive method to analyze viral spike N-glycoforms, assessing glycan occupancy and processing from high-mannose to complex forms at each N-glycosylation site.

Vaccine Elicitation of HIV Broadly Neutralizing Antibodies from Engineered B cells

This study shows that engineered HIV bnAb-expressing B cells can be expanded in vivo after vaccination, leading to durable bnAb memory and long-lived plasma cells.

Targeting HIV Env immunogens to B cell follicles in non-human primates through immune complex or protein nanoparticle formulations

This study shows that HIV Env immunogens can be concentrated in B cell follicles using immune complexes or protein nanoparticles, enhancing their localization in lymph nodes and potentially improving immune responses.

HIV-1 Envelope and MPER antibody structures in lipid assemblies

This study presents methods to incorporate full-length HIV-1 Env into lipid assemblies for structural analysis, revealing the quaternary epitope of an MPER antibody and Env tilting during neutralization.