J. D. Allen

Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines

The paper introduces a triple tandem trimer (TTT) design for HIV-1 Env and influenza HA immunogens, showing improved trimer-specific immune responses in viral vectored vaccines.

Focusing antibody responses to the fusion peptide in rhesusmacaques

This study uses continuous immunogen delivery in rhesus macaques to focus antibody responses on the HIV fusion peptide, identifying key residues for vaccine design.

Mechanisms of SARS-CoV-2 Inactivation using UVC Laser Radiation

This study investigates the inactivation of SARS-CoV-2 using UVC laser radiation and identifies mechanisms involving RNA damage and disruption of spike protein function.

The diversity of the glycan shield of sarbecoviruses closely related to SARS-CoV-2

This study compares the glycan shields of sarbecoviruses to understand how sequence variations affect their structure and potential for antibody neutralization.

Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimers as HIV-1 vaccine candidates

Glycan trimming of HIV-1 Env immunogens improves vaccine-induced neutralizing antibody responses in small animals and primates.

Molecular insights into antibody-mediated protection against the prototypic simian immunodeficiency virus

This study identifies nAbs against SIVmac239, reveals structural features of its Env trimer, and shows passive transfer of nAbs protects macaques from infection.

Structure of the hepatitis C virus E1E2 glycoprotein complex

The paper presents the cryo-EM structure of the HCV E1E2 glycoprotein complex with antibodies, revealing the E1-E2 interface and its stabilization mechanisms.

Site-specific steric control of SARS-CoV-2 spike glycosylation

The paper compares glycosylation patterns of recombinant SARS-CoV-2 spike proteins across labs with infectious virus S protein, finding conserved site-specific glycan maturation stalling linked to protein structure.

Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM

The paper uses cryoEM to analyze polyclonal antibody responses to HIV Env immunogens in rhesus macaques, revealing structural differences in antibody binding and immunogenicity of engineered mutations and glycan sites.

Native-like SARS-CoV-2 spike glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine

This study characterizes the structure, conformation, and glycosylation of the SARS-CoV-2 spike protein expressed by the ChAdOx1 nCoV-19/AZD1222 vaccine, confirming its native-like properties.

Human neutralizing antibodies against SARS-CoV-2 require intact Fc effector functions and monocytes for optimal therapeutic protection

This study shows that neutralizing antibodies against SARS-CoV-2 need intact Fc functions and monocytes for optimal therapeutic protection in animal models.

Neutralizing antibodies induced by first-generation gp41-stabilized HIV-1 envelope trimers and nanoparticles

This study isolates HIV-1 neutralizing antibodies from mice and rabbits immunized with gp41-stabilized trimers and nanoparticles, showing they target different epitopes and elicit Env-dependent breadth.

Immunofocusing and enhancing autologous Tier-2 HIV-1 neutralization by displaying Env trimers on two-component protein nanoparticles

This study shows that displaying HIV-1 Env trimers on two-component protein nanoparticles enhances autologous Tier-2 neutralizing antibody titers in rabbits.

Two-component spike nanoparticle vaccine protects macaques from SARS-CoV-2 infection

This paper develops a two-component spike nanoparticle vaccine that protects macaques from SARS-CoV-2 infection by inducing strong neutralizing antibodies and reducing viral replication.

Enhancing glycan occupancy of soluble HIV-1 envelope trimers to mimic the native viral spike

The paper modifies HIV-1 Env trimers to increase glycan occupancy and reduce artificial glycan holes, improving vaccine antigen design.

Site-specific analysis of the SARS-CoV-2 glycan shield

The paper uses mass spectrometry to analyze glycan structures on the SARS-CoV-2 spike protein, revealing differences from host glycan processing and implications for vaccine design.

Networks of HIV-1 envelope glycans maintain antibody epitopes in the face of glycan additions and deletions

The paper investigates how HIV-1 envelope glycan networks affect the formation and stability of broadly neutralizing antibody epitopes, showing their importance in immunogen design.

Vulnerabilities in coronavirus glycan shields despite extensive glycosylation

This study identifies vulnerabilities in coronavirus glycan shields by showing that MERS S protein has under-processed oligomannose-type glycan clusters, unlike SARS and HKU1 CoVs, and that these glycans do not form an effective shield against antibodies.

Structural and functional evaluation of de novo-designed, two-component nanoparticle carriers for HIV Env trimer immunogens

The paper evaluates de novo-designed two-component nanoparticles for presenting HIV Env trimers, showing improved antibody responses through multivalent display.

Similarities and differences between native HIV-1 envelope glycoprotein trimers and stabilized soluble trimer mimetics

This study compares native HIV-1 Env trimers with soluble SOSIP trimers, finding structural and antigenic similarities but differences in antibody binding and glycan composition.