W. R. Schief

Diverse competitor B cell responses to a germline-targeting priming immunogen in human Ig loci transgenic mice

The paper analyzes B cell responses to a germline-targeting immunogen in human Ig loci transgenic mice, finding diverse non-VRC01-class B cells and competitor B cells recognizing epitopes near the VRC01 epitope.

Humanized V(D)J-rearranging and TdT-expressing Mouse Vaccine Models with Physiological HIV-1 Broadly Neutralizing Antibody Precursors

This paper introduces new mouse models expressing human HIV-1 broadly neutralizing antibody (bnAb) precursors with more physiological levels and diversity, enhancing vaccine research for bnAb elicitation.

Use of Transient Transfection for cGMP Manufacturing of eOD-GT8 60mer, a Self-Assembling Nanoparticle Germline-Targeting HIV-1 Vaccine Candidate

This paper describes the cGMP production and characterization of eOD-GT8 60mer, a self-assembling HIV-1 vaccine nanoparticle, using transient transfection in HEK293 cells and subsequent purification techniques.

IL-2 secreting T helper cells promote EF B cell maturation via intrinsic regulation of B cell mTOR/AKT/Blimp-1 axis.

The paper identifies IL-2's role in promoting B cell maturation by regulating the mTOR/AKT/Blimp-1 axis through T helper cell interactions.

Efficient isolation of rare B cells using next-generation antigen barcoding

This paper introduces a method for efficiently isolating rare antigen-specific B cells using next-generation antigen barcoding and a computational framework, demonstrating its ability to recover thousands of mAbs, including rare HIV broadly neutralizing antibodies.

Application of B cell immortalization for the isolation of antibodies and B cell clones from vaccine and infection settings

This study applies Bcl-6/Bcl-xL retroviral transduction to isolate antibodies and generate B cell clones from various B cell populations in healthy and infected individuals.

A broad and potent neutralization epitope in SARS-related coronaviruses

The paper identifies a broadly neutralizing epitope in SARS-related coronaviruses that is effective against variants, including Omicron, and suggests its use for universal vaccine design.

Long-lasting germinal center responses to a priming immunization with continuous proliferation and somatic mutation

This study shows that germinal center B cells can persist and continue to mutate for 29 weeks after a single immunization, leading to long-lasting antibody responses.

Spatially regulated protease activity in lymph nodes renders B cell follicles a sanctuary for retention of intact antigens

The paper shows that spatially restricted protease activity in lymph node follicles preserves antigen integrity, leading to stronger GC responses when antigens are targeted to follicular dendritic cells.

DeGlyPHER: an ultrasensitive method for analysis of viral spike N-glycoforms

DeGlyPHER is an ultrasensitive method to analyze viral spike N-glycoforms, assessing glycan occupancy and processing from high-mannose to complex forms at each N-glycosylation site.

From Structure to Sequence: Identification of polyclonal antibody families using cryoEM

This paper uses cryoEM and next-generation sequencing to identify monoclonal antibodies from polyclonal sera and validate their antigen-binding properties.

Vaccine genetics of IGHV1-2 VRC01-class broadly neutralizing antibody precursor naive human B cells

The paper screens naive B cells for VRC01-class broadly neutralizing antibody precursors using a germline-targeting immunogen and single-cell BCR sequencing, finding common germline variation affecting gene usage.

Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM

The paper uses cryoEM to analyze polyclonal antibody responses to HIV Env immunogens in rhesus macaques, revealing structural differences in antibody binding and immunogenicity of engineered mutations and glycan sites.

Controlling wireframe DNA origami nuclease degradation with minor groove binders

This study demonstrates that minor groove binders can protect wireframe DNA origami from endonuclease degradation, enhancing their stability in biological environments.

CoV3D: A database and resource for high resolution coronavirus protein structures

CoV3D provides a database of coronavirus protein structures, including spike glycoprotein complexes, for vaccine and therapeutic design.

B cells expressing authentic naive human VRC01-class BCRs can be primed and recruited to germinal centers in multiple independent mouse models

The paper demonstrates that authentic naive human VRC01-class BCRs can be primed and recruited to germinal centers in multiple mouse models, validating germline-targeting vaccine design.

Targeting HIV Env immunogens to B cell follicles in non-human primates through immune complex or protein nanoparticle formulations

This study shows that HIV Env immunogens can be concentrated in B cell follicles using immune complexes or protein nanoparticles, enhancing their localization in lymph nodes and potentially improving immune responses.

Role of nanoscale antigen organization on B-cell activation probed using DNA origami

This study uses DNA origami to investigate how antigen organization affects B-cell activation, finding that optimal spacing and valency enhance signaling.

HIV-1 Envelope and MPER antibody structures in lipid assemblies

This study presents methods to incorporate full-length HIV-1 Env into lipid assemblies for structural analysis, revealing the quaternary epitope of an MPER antibody and Env tilting during neutralization.

Vaccine-induced protection from homologous Tier 2 simian-human immunodeficiency virus challenge in nonhuman primates

This study shows that high serum neutralizing antibody titers from Env protein vaccination protect rhesus macaques from Tier 2 SHIV challenge, with ~1:500 ID50 titers providing over 90% protection.

Slow delivery immunization enhances HIV neutralizing antibody and germinal center responses via modulation of immunodominance

This study shows that slow delivery immunization in rhesus macaques increases HIV-neutralizing antibody titers and germinal center responses by modulating immunodominance.

RosettaAntibodyDesign (RAbD): A General Framework for Computational Antibody Design

This paper introduces RosettaAntibodyDesign (RAbD), a computational framework for antibody design that improves affinity by redesigning CDRs using structural and sequence-based methods.