Zebrafish Biomedical Research Applications

The paper introduces a method to raise individual juvenile zebrafish in a recirculating system for experiments requiring repeated measures, showing growth comparable to conventional methods.

The paper identifies novel tagln-positive mural cell populations in zebrafish embryos using transgenic markers and reveals their developmental contributions.

This study identifies BMP, FGF, Notch, and Shh signaling pathways as critical for gill regeneration in zebrafish following filament resection.

This paper develops a high-throughput method to count EGFP-expressing neutrophils in zebrafish larvae using a parallelized microscope and machine learning, validated against manual counts.

This study shows that warming reduces routine swimming in larval zebrafish and alters molecular responses to novel odours, with energy trade-offs in gene expression.

The paper investigates the distinct roles of core autophagy-related genes in zebrafish definitive hematopoiesis, revealing gene-specific and synergistic effects on hematopoietic stem cells and myeloid cell types.

The paper introduces an all-optical technique for brain-wide calcium imaging and optogenetic stimulation in larval zebrafish, showing it can trigger behavior and neural activity changes.

This paper introduces KimmelNet, a deep learning model that predicts zebrafish embryo age from 2D brightfield images and detects developmental delay between populations.

The paper develops a robust strategy for generating knock-in reporter animals using minicircle technology and an ectopic promoter, improving efficiency and reducing unintended genomic effects.

The paper introduces a low-cost, open-source system for tracking larval zebrafish behavior during functional microscopy.

The paper evaluates the effects of 162 FDA-approved drugs on zebrafish embryogenesis and immune response to SARS-CoV-2 spike protein, identifying potential developmental side effects.

This study shows adgrl3.1-deficient zebrafish exhibit externalizing behaviors rescued by atomoxetine, and identifies differentially expressed genes linked to externalizing disorders.

This study shows adult zebrafish can regenerate jaw joint ligaments without scarring through dedifferentiation of ligamentocytes and ECM remodeling, with legumain playing a key role.

This study identifies the mesencephalic locomotor region's role in recruiting V2a reticulospinal neurons to control forward locomotion in zebrafish.

The paper shows that endothelial cells from the dermomyotome contribute to the dorsal aorta and support hematopoietic stem cell emergence in zebrafish.

This study investigates how varying acute stressor intensity affects social approach and maintenance in adult zebrafish, showing intensity-dependent reductions in both behaviors with social maintenance affected at lower stress levels.

The paper develops genetic tools for the mangrove killifish, improving embryo yield and enabling targeted gene knockout studies.

The paper identifies a macrophage subpopulation that mediates airineme-mediated signaling through MMP9-dependent mechanisms in zebrafish.

OptoPi is an open-source platform for small animal behaviour analysis with custom illumination and optogenetic stimulation, demonstrating tracking of zebrafish and Drosophila larvae.

The paper identifies IGFR/mTOR signaling as a driver of increased translation during zebrafish fin regeneration, accelerating outgrowth through coordinated cell behavior.

The paper shows that modulating Embryonic Neuronal Excitability (ENE) in zebrafish embryos affects dopaminergic neuron numbers and larval locomotion, with implications for neurodevelopmental disorders.

The paper examines the effects of caldesmon mutations in zebrafish embryos, finding mild developmental impacts and normal gross development, suggesting caldesmon may be a viable cancer therapy target.

This study identifies the role of progenitor-derived glia in zebrafish spinal cord regeneration through genetic and molecular analyses.

This study investigates the role of 5-HT2C receptors in zebrafish social behavior using pharmacological manipulations and behavioral tests.

This study shows that early-life stress in zebrafish affects anxiety-related behaviors and these effects are transgenerational across three generations.

This study shows that arnt1 and arnt2 genes are required alongside npas4l for hemato-vascular specification in zebrafish embryos, with each gene necessary but not sufficient alone.

This study examines the effects of quinpirole, a dopamine D2/D3 receptor agonist, on behavior and neurotransmitter levels in adult zebrafish, finding altered locomotion, anxiety, memory, and neurotransmitter profiles.

The paper introduces an efficient CRISPR-Cas9-based knock-in method in zebrafish for lineage tracing and generates reporter lines for specific cell types.

The paper describes how ZFIN annotates phenotype, expression, and disease model data in zebrafish research across different experimental designs.

The paper identifies three distinct neural pathways in larval zebrafish that mediate habituation to dark looming stimuli, with one pathway specifically inhibiting escape responses.