Plant biochemistry and biosynthesis

This study characterizes WsCYP71B35, a cytochrome P450 enzyme in Ashwagandha, showing its role in withanolides biosynthesis and bacterial defense.

This paper develops a sustainable method to produce agarwood-like sesquiterpenoids using synthetic biology in algae, avoiding the need for harvesting endangered trees.

The paper investigates how ketocarotenoid production in tomato triggers metabolic reprogramming and cellular adaptation to maintain homeostasis.

The paper identifies allelic variation in PSY and DXS genes associated with carotenoid content and fruit color intensity in red chilli peppers.

The paper annotates and explores variation in cannabinoid biosynthesis genes across multiple Cannabis sativa genomes, identifying copy number variation and exon usage differences.

This study elucidates the biosynthesis of unique benzylisoquinoline alkaloids in sacred lotus and enables their microbial synthesis.

This study shows that fusing terpene synthases increases metabolic flux by boosting enzyme stability and expression, leading to higher nerolidol production.

The paper presents a chromosome-scale genome assembly of Jasminum sambac var. unifoliatum to study the genetic basis of floral fragrance production.

The paper analyzes copy number variations in Arabidopsis thaliana metabolic gene clusters and links them to climatic adaptation.

This study identifies non-Cannabis BBE superfamily enzymes that catalyze CBGA to CBSA, opening new avenues for cannabinoid biosynthesis.

This study improves yeast-based biosynthesis of monoterpenoid indole alkaloids by expressing a key enzyme and integrating biosynthetic gene cassettes, enabling production of vinblastine precursors and their unnatural derivatives.

This study shows overexpression of PtDXR and PtHMGR in poplars alters gene expression and isoprenoid levels via MEP and MVA pathways.

The paper reconstitutes strictosidine biosynthesis in Nicotiana benthamiana by co-expressing 14 enzymes, with MLPL from Nepeta enhancing flux through the iridoid pathway.

The paper reports a high-quality genome of Isochrysis galbana and uses multi-omics analysis to identify genes and pathways involved in fucoxanthin biosynthesis under green light conditions.

This paper develops a microbial consortium to synthesize strigolactones and identifies plant enzymes involved in their biosynthesis.

The paper presents a chromosome-level genome of Taxus chinensis var. mairei, revealing unique genome evolution and genetic structures for paclitaxel biosynthesis.

This study identifies how ATP drives efficient terpene biosynthesis in marine thraustochytrids by enhancing thermodynamic driving force through energy metabolism shifts.

The paper predicts precursor cation specificity in plant sesquiterpene synthases using machine learning and co-evolution analysis, identifying key residues for enzyme function.

This paper demonstrates the feasibility of engineering 2-phenylethylglucosinolate production in Nicotiana benthamiana by combining biosynthetic genes from Barbarea vulgaris and Arabidopsis thaliana, resulting in increased 2PE yield through chimeric pathway optimization.

This paper describes the production of moth sex pheromones in transgenic plants and evaluates their release rates.

This study overexpresses HMGR and SQE enzymes in diatoms to alter sterol profiles, showing differential accumulation of intermediates and end-point sterols in Phaeodactylum tricornutum.

This study improves taxadiene production in Saccharomyces cerevisiae by engineering TASY solubility and optimizing bioprocess variables, achieving up to 129 mg/L at 20°C.

This paper reviews strategies to improve triterpenoid biosynthesis in yeast by engineering enzymes and metabolism, achieving gram-scale production from milligram-scale levels.

This study identifies RCP2, a TPR protein regulating carotenoid biosynthesis and chromoplast development in monkeyflowers, showing its independent role from RCP1.

The paper analyzes plant sesquiterpene synthases to identify motifs and unexplored sequence regions, and demonstrates a database application for guiding mutagenesis studies on product specificity.

This paper describes the engineering of novel halogenated indigo precursors in plants using a combinatorial pathway, achieving significant metabolite production and validation.