Porphyrin Metabolism and Disorders

Glycine betaine-mediated transcriptional stimulation links its catabolism and vitamin B12 biosynthesis in Rhizobiaceae

The paper shows glycine betaine activates transcription of its catabolic pathway and vitamin B12 biosynthesis genes in Rhizobiaceae, linking methyl group supply to biosynthetic output.

Isolation and In vitro Characterization of BchE, the Cobalamin-Dependent Anaerobic Magnesium Protoporphyrin IX Monomethylester Cyclase Involved in Bacteriochlorophyll Biosynthesis

This paper isolates and characterizes BchE, a cobalamin-dependent radical SAM enzyme, enabling in vitro studies of its role in bacteriochlorophyll biosynthesis.

Cellular basis of B12 uptake and remodelling in microalgae revealed using a novel bioassay

The paper develops a novel bioassay to quantify B12 vitamers in microalgae and identifies proteins involved in B12 uptake and remodelling.

An adaptor for feedback regulation of heme biosynthesis by the mitochondrial protease CLPXP

The paper identifies POLDIP2 as a heme-sensing adaptor that facilitates CLPXP-mediated degradation of ALAS, elucidating the mechanism of heme-induced feedback regulation in heme biosynthesis.

Assembly of the ATP-driven cobalt chelatase

The paper investigates the step-wise assembly mechanism of the ATP-driven cobalt chelatase CobSTN involved in vitamin B12 biosynthesis.

Environment-dependent landscapes of coding variant impacts on coproporphyrinogen oxidase

The paper systematically assesses the functional impact of CPOX variants under different environmental conditions, revealing mercury-dependent variant effects and improving variant classification.

Evolutionarily divergent DUF4465 domains have a common vitamin B12-binding function

The paper identifies B12-binding function in multiple DUF4465 proteins across diverse bacterial species.

A reversible feedback mechanism regulating mitochondrial heme synthesis

This study identifies a reversible feedback mechanism where heme inhibits mature mitochondrial ALAS2, regulating heme synthesis and providing insights into its spatial control.

Microbes display broad diversity in cobamide preferences

The paper characterizes bacterial cobamide preferences by testing growth under specific cobamide-dependent conditions, revealing broad diversity in cobamide usage across organisms and metabolic pathways.

Systematically testing human HMBS missense variants to reveal mechanism and pathogenic variation

This study systematically tests HMBS missense variants to determine their functional impact and distinguish pathogenic from benign variants.

Zebrafish models for human ALA-dehydratase-deficient porphyria (ADP) and hereditary coproporphyria (HCP) generated with TALEN and CRISPR-Cas9

This paper generates zebrafish models for human ALA-dehydratase-deficient porphyria and hereditary coproporphyria using TALEN and CRISPR-Cas9, revealing phenotypes similar to human diseases.