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LigHTS: Massively Parallel Biomimetic Photo-Functionalization for Imaging-Based Ultra-High-Throughput Screening

This paper introduces LigHTS, a method to create biomimetic hydrogels in standard microplates for high-throughput screening, enabling tunable thickness and topography with improved reproducibility.

Surfactant-Enhanced Hybridization Capture for Direct Sequence-Specific RNA Extraction from Plasma and Whole Blood

This paper introduces a surfactant-based method for direct RNA extraction from plasma and whole blood, achieving high recovery of low-abundance RNA and outperforming existing silica-based kits.

Coordinated Enhancement of Molecular and Spatial Fidelity for Biological Inference in Spatial Transcriptomics

FOCUS improves spatial transcriptomics by coordinating molecular and spatial fidelity across multiple tasks and datasets, revealing clearer biological patterns in immunology, development, and oncology.

Antiviral Sulfonated Polymersomes Inhibit Corona-, Influenza-, and Alphavirus

This study designs sulfonated polymersomes that inhibit multiple viruses by targeting host cell receptors, showing potent in vitro and in vivo antiviral activity.

Rational Design of 5' UTRs for Optimized mRNA Expression and Cost-Effective, Rapid Analysis of Capped mRNA

This paper designs a 5' UTR that enables rapid, cost-effective analysis of mRNA capping efficiency and poly(A) tail length without probes or biotin.

Design and characterization of peptide-responsive biosensors

This study designs and characterizes peptide-responsive biosensors that regulate gene expression using intracellular peptide effectors and transcription factors in vitro and in vivo.

Fibrosis burden and spatial pattern affect left atrial appendage stasis in multi-beat multi-physics simulations

This study investigates how fibrosis burden and spatial distribution affect left atrial appendage hemodynamics using multi-physics simulations.

3D-PAULM: Integrated Photoacoustic Tomography and Ultrasound Localization Microscopy for Multiparametric Brain and Tumor Imaging

This paper introduces 3D-PAULM, an integrated imaging platform that combines photoacoustic tomography and ultrasound localization microscopy to enable multiparametric brain and tumor imaging.

Evaluation of Iodine-Based Contrast Agents for Ex Vivo Micro-CT Imaging of Porcine Hearts: An Application to Cardiac Conduction System Imaging

This study compares two iodine-based contrast agents for micro-CT imaging of porcine hearts, finding that I2E provides better contrast and preservation while I2KI offers faster staining but more artifacts.

Quantitative 3D imaging of intact engineered heart tissues using inverted selective plane illumination microscopy

This paper develops an inverted selective plane illumination microscopy platform to quantitatively image and analyze 3D engineered heart tissues, revealing structural features and heterogeneities.

LATEER: Low-Cost Open-Source Platform for Electrical Stimulation and TEER Measurement in Human Cardiomyocytes

LATEER is a low-cost, open-source platform for electrical stimulation and TEER measurement in human cardiomyocytes, validated with human pluripotent stem cell-derived cardiomyocytes and mesenchymal stem cells.

A minimally modified AAV9 variant (AAV9fyhr) for enhanced CNS gene delivery by intra-CSF administration.

This paper describes a novel AAV9 variant, AAV9fyhr, that improves CNS gene delivery while minimizing peripheral tissue tropism through targeted amino acid modifications.

Predicting viral host range for reverse zoonosis surveillance

The paper predicts viral host range using a combination of experimental screening and deep learning to identify potential animal reservoirs for reverse zoonotic transmission.

Biohybrid toggle switches: leveraging bistability in muscle-actuated machines

This paper introduces a biohybrid bistable switching mechanism using skeletal muscle to achieve energy-efficient, reversible toggling between states, demonstrating its potential as an electromechanical switch in robotics.

Bioengineered human bone-mimetic niche compositions regulatebreast cancer cell quiescence and therapy response

This study develops a 3D human bone-mimetic niche system to investigate how different bone compartments regulate breast cancer cell quiescence and therapy response.

MINT: Predicting Enzyme-Cofactor Specificity by Multimodal Knowledge Transfer from Protein-Ligand Interactions

MINT predicts enzyme-cofactor specificity using a multimodal framework with transfer learning, achieving high accuracy in cofactor binding prediction.

Evaluation of Densified Collagen Tubes as an Interposition Graft in Abdominal Aorta

This study evaluates densified collagen tubes as interposition grafts in rat abdominal aorta, showing patent grafts for 17 weeks with neointima formation and re-endothelialization but late thrombosis.

Large-Scale Mutagenesis Identifies Sequence Changes That Bias Tau Toward CTE-like Aggregation

The paper identifies sequence changes that bias tau aggregation toward CTE-like structures using mutagenesis and structural analysis.

Unsupervised 3D classification and characterisation of osteocyte and chondrocyte lacunae in the murine osteochondral unit

This paper presents an automated pipeline to classify osteocyte and chondrocyte lacunae in mouse osteochondral units using microCT, achieving high accuracy without manual intervention or cement line identification.

A bioengineered model of human placental exposure to environmental metals during pregnancy

This paper develops a bioengineered model of the human placenta to study metal exposure during pregnancy and identifies protective roles of efflux transporters and potential metabolic biomarkers.