Discover, rate, and discuss biology preprints.

This study identifies how genetic and environmental factors shape the human immune cell epigenome through differential methylation and chromatin accessibility changes.

This study uses pan-genome and multi-omics analyses to investigate the genomic basis of mycoheterotrophy in Gastrodia elata, revealing gene loss, regulatory co-option, and specialized nutrient acquisition mechanisms.

The paper analyzes azithromycin resistance mutations in Chlamydia trachomatis genomes, finding common mutations in rplV that predate azithromycin's clinical use.

The paper uses single-cell RNA sequencing to analyze somatic mutations in HSCs and finds age-related clonal expansion and transcriptional divergence.

The paper shows that polygenic backgrounds affect the phenotypic impact of genetic variants across different levels, suggesting that selection coefficients vary with genetic context.

This paper presents a chromosome-level genome assembly and annotation for the degu, improving contiguity, completeness, and gene annotation compared to previous assemblies.

This study uses whole-genome sequencing to show that captive black soldier fly populations have reduced genetic diversity and inbreeding due to human-mediated dispersal and uncoordinated breeding.

The paper presents the telomere-to-telomere genome of Xiphinema index, revealing HGTs related to plant parasitism and an unprecedented epigenetic toolkit in nematodes.

This study identifies reciprocal selective retention of transcription factors in Upland and Sea Island cotton, explaining the yield-quality trade-off through genomic and transcriptomic analyses.

The paper investigates how alternative splicing of IKZF1 affects gene regulation in T cells, finding significant changes in gene expression and chromatin accessibility.

The paper evaluates a SNP panel's ability to detect hybridization across generations in European lobsters using resampling-based methods and empirical data.

The paper evaluates four TIS sequencing library generation protocols to reduce costs and improve efficiency for gene essentiality analysis in E. coli.

This study resequences sable and pine marten genomes to reveal hybridization patterns, fertility, and genetic diversity, identifying candidate genes and divergence timing.

The paper corrects a strain mislabeling error in a genome sequencing study of Prymnesium parvum, highlighting the need for clade-specific markers.

The paper shows classical enhancers influence 3D genome structure by linking DNA sequence to transcriptional condensates and chromatin organization through specific transcription factors.

The paper uses phylogenomic analysis to study reticulate evolution in the Malvatheca clade, finding evidence of ancient hybridization and polyploidy affecting subfamilial classification.

The paper introduces the PC-index, a composite metric that integrates gene expression magnitude and prevalence in single-cell RNA-seq data, demonstrating its application in human adipocytes.

The paper builds a cross-species single-cell multi-omics kidney atlas to identify epithelial cells as a driver of epigenetic aging in diseased kidneys.

This study compares two single-cell RNA-sequencing multiplexing techniques, 10x and Parse, on cerebellar organoids, finding similar cellular diversity but differences in transcript capture and cell stress.