Author

A. S. Puschnik

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Microbiology· 4 Bioengineering· 1 Cell Biology· 1

Signature Assays, Organisms & Methods

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Approach:genetic perturbation / CRISPR· 4 Research Focus:host-pathogen & immune response· 3 Organism:human· 3 Approach:experimental assay· 2 Assay:proteomics· 2 Research Focus:cell signaling & trafficking· 1 Research Focus:cell type annotation· 1 Assay:CRISPR screen· 1

Frequent Co-Authors

J. Kulsuptrakul· 3 shared M. Ott· 2 shared R. Wang· 2 shared A. M. Ngo· 1 shared C. M. Worthington· 1 shared C. R. Simoneau· 1 shared C. Y. Wang· 1 shared D. Tran· 1 shared

Activity by Year

2019-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 12 mutual co-author links

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3 shared papersMicrobiology

J. Kulsuptrakul

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Author profile →

6 papers

Recent·Most cited

Open-source milligram-scale, four channel, automated protein purification system

R. R. Puccinelli, S. S. Sama, C. M. Worthington, A. S. Puschnik, J. E. Pak, R. Gomez-Sjoberg

The paper describes an open-source automated protein purification system capable of parallelizing milligram-scale purification of multiple recombinant proteins.

bioRxiv · Aug 9, 2023Bioengineering

SARS-CoV-2 Envelope-mediated Golgi pH dysregulation interferes with ERAAP retention in cells

V. Vargas-Zapata, K. M. Geiger, D. Tran, J. Ma, X. Mao, A. S. Puschnik, L. Coscoy

This study identifies SARS-CoV-2 Envelope protein-induced Golgi pH neutralization as a mechanism causing ERAAP loss and extracellular release, highlighting ERAAP as a sensor of secretory pathway disturbances during infection.

bioRxiv · Nov 29, 2022 · PreprintCell Biology

Human sialomucin CD164 is an essential entry factor for lymphocytic choriomeningitis virus

J. Liu, K. A. Knopp, E. Rackaityte, C. Y. Wang, M. T. Laurie, S. Sunshine, A. S. Puschnik, J. DeRisi

This study identifies CD164 as an essential entry factor for LCMV in human cells and tissues, showing its role in viral infection and potential as a therapeutic target.

bioRxiv · Jan 24, 2022 · PreprintMicrobiology

Functional genomic screens identify human host factors for SARS-CoV-2 and common cold coronaviruses

R. Wang, C. R. Simoneau, J. Kulsuptrakul, M. Bouhaddou, K. Travisano, J. M. Hayashi, J. Carlson-Stevermer, J. Oki, K. Holden, N. J. Krogan, M. Ott, A. S. Puschnik

The paper identifies host factors and pathways common to SARS-CoV-2 and common cold coronaviruses using CRISPR screens, revealing shared and unique factors that support viral infection.

bioRxiv · Sep 24, 2020Microbiology

A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection

J. Kulsuptrakul, R. Wang, N. L. Meyers, M. Ott, A. S. Puschnik

bioRxiv · Dec 15, 2019Microbiology

The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins

A. M. Ngo, M. J. Shurtleff, K. D. Popova, J. Kulsuptrakul, J. S. Weissman, A. S. Puschnik

This study shows the ER membrane protein complex is essential for correct topology and stable expression of flavivirus polyproteins by preventing degradation of NS4A-NS4B.

bioRxiv · Sep 4, 2019Microbiology