T. Saito

Structural and Dynamic Insights into the Biased Signaling Mechanism of the Human Kappa Opioid Receptor

This study investigates the structural dynamics of the human KOR-Gi signaling complex with G-protein-biased and balanced agonists, identifying key residues involved in signaling bias.

Can Colpoda travel across oceans? Salinity tolerance of resting cysts may enable global dispersal of the species.

The study investigates whether Colpoda cucullus resting cysts can tolerate saline conditions, suggesting a mechanism for oceanic dispersal of freshwater protists.

NEDD4-binding protein 1 suppresses HBV replication by degrading HBV RNAs

This paper identifies N4BP1 as a host factor that suppresses HBV replication by degrading HBV RNAs, with its KH-like and RNase domains required for this effect.

Glucose stockpile in the intestinal apical brush border in C. elegans

The paper measures glucose diffusion in C. elegans intestinal brush border, finding it significantly slower than in bulk, suggesting a glucose stockpile in this viscous layer.

Eosinophils exert direct and indirect anti-tumorigenic effects in the development of esophageal squamous cell carcinoma

This study shows eosinophils reduce esophageal squamous cell carcinoma by releasing reactive oxygen species and inhibiting IL-17 pathways.

Subchronic Toxicity Assessment of Perfluoroalkyl Substances by Mixed Exposure of Nine Substances at Daily Intake Relevant Concentration

This study assesses the subchronic toxicity of a PFAS mixture in mice, finding liver morphological changes and alterations in metabolism and cancer-related gene expression.

Exon 44 skipping in Duchenne muscular dystrophy: NS-089/NCNP-02, a dual-targeting antisense oligonucleotide

This study develops NS-089/NCNP-02, a dual-targeting ASO for exon 44 skipping in DMD, showing effective in vitro and in vivo exon skipping and dystrophin expression.

Morphogenesis of bullet-shaped rabies virus particles requires a functional interplay between the viral matrix protein and ESCRT-I component TSG101

This study identifies the interaction between rabies virus matrix protein and TSG101 as essential for bullet-shaped virion formation and efficient budding.

Single-Cell Imaging Maps Inflammatory Cell Subsets to Pulmonary Arterial Hypertension Vasculopathy

The paper maps immune cell subsets in PAH lungs using MIBI-TOF, identifying mo-DCs and their role in vascular remodeling and linking immune dysregulation to disease severity in HPAH.

Novel therapeutic strategies for injured endometrium: Autologous intrauterine transplantation of menstrual blood-derived cells from infertile patients

This study evaluates the therapeutic potential of menstrual blood-derived cells from infertile patients for injured endometrium, showing improved endometrial thickness and fertility outcomes in a mouse model.

Necessity of integrated genomic analysis to establish a designed knock-in mouse from CRISPR-Cas9-induced mutants

The paper demonstrates that integrated genomic analysis is necessary to accurately identify and establish a designed knock-in mouse strain from CRISPR-Cas9-induced mutants.

Analysis of chemical and mechanical behaviors in living cells by continuum mechanics-based FRAP

This study develops a continuum mechanics-based model to analyze long-term FRAP responses, revealing spatially distinct molecular turnover rates and mechanical properties in living cells.

Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cells

This study identifies Map7D2 and Map7D1 as regulators of microtubule stability in neuronal cells, showing they function through distinct mechanisms.

Differential Binding Kinetics for Evaluating Immune Checkpoint Inhibitors in Serum

This study measures binding kinetics of immune checkpoint inhibitors in serum using GMR biosensors and compares them to label-free methods, finding serum affects off-rates and pharmacological modeling.

Microglia and border-associated mouse macrophages maintain their embryonic origin during Alzheimers disease

This study shows that microglia and border-associated macrophages in Alzheimer's disease are not replenished by bone marrow-derived cells, maintaining their embryonic origin.

Long-term molecular turnover of actin stress fibers revealed by advection-reaction analysis in fluorescence recovery after photobleaching

This study develops a new FRAP model to analyze long-term molecular turnover, revealing that advection and chemical reaction rates are comparable in actin stress fibers.

Effect of salinity and water dilution on environmental DNA degradation in freshwater environments

This study examines how salinity and water dilution affect eDNA degradation in pond water, finding slower degradation rates in saline and diluted conditions.

Host EPAC1 modulates rickettsial adhesion to vascular endothelial cells via regulation of ANXA2 Y23 phosphorylation

This study investigates how EPAC1 regulates rickettsial adhesion to endothelial cells by modulating ANXA2 Y23 phosphorylation, using in vivo models and FluidFM.

THz irradiation inhibits cell division by affecting actin dynamics

This study shows THz irradiation inhibits cell division by preventing the disappearance of the contractile ring and promoting F-actin polymerization in human cells.

Environmental DNA degradation simulation from water temperature and DNA fragment length: A meta-analysis approach

This study simulates eDNA degradation using water temperature and fragment length from 28 studies, finding significant relationships and predicting degradation rates.

On-site eDNA detection of species using ultra-rapid mobile PCR

This study develops an ultra-rapid mobile PCR method for on-site eDNA detection of aquatic species, demonstrating high detectability and faster results than traditional lab-based approaches.

Determining the domain-level reaction-diffusion properties of cellular proteins by incorporating genetic engineering to FRAP-based framework

This paper introduces a FRAP-based framework with genetic engineering to determine domain-level reaction and diffusion properties of proteins, demonstrating its application to transgelin-2.

Antihypertensive effect of brain-targeted mechanical intervention with passive head motion

Passive head motion reduces blood pressure in hypertensive rats and humans by decreasing AT1R expression in RVLM astrocytes through interstitial fluid shear stress.

Suppression of amyloid-β secretion from neurons by cis-9, trans-11-octadecadienoic acid, an isomer of conjugated linoleic acid

This study shows that c9,t11 CLA reduces amyloid-beta production in neurons by altering BACE1 and APP localization, potentially protecting against neurodegeneration.

Degradation modeling of water environmental DNA: Experiments on multiple DNA sources in pond and seawater

The paper experiments on eDNA degradation in pond and seawater, finding exponential decay for cell- and fragmental DNA-derived eDNA and validating an exponential degradation model.

Annexin A2 depletion exacerbates the intracerebral microhemorrhage induced by acute rickettsia and Ebola virus infections

This study shows that ANXA2 depletion increases CMHs in rickettsia and Ebola virus infections by disrupting BBB tight junctions.

Disrupted remapping of place cells and grid cells in knock-in model of Alzheimer’s disease

This study shows that in a knock-in Alzheimer’s disease model, remapping of place and grid cells is disrupted, leading to impaired spatial memory circuits.

Autophagy controls lipid droplet formation by fine-tuning NCoR1 levels

This study shows autophagy regulates lipid droplet formation by modulating NCoR1 levels, affecting LXR target gene expression and lipid metabolism in the liver.