Dorota Klepacki

Structural modification of oxazolidinone antibiotics alters nascent peptide stalling preference and peptide trajectory through the ribosome

This study shows that structural modifications of oxazolidinone antibiotics alter their preference for specific penultimate amino acids during translation inhibition.

Sequence-specific trapping of EF-Tu/glycyl-tRNA complex on the ribosome by bottromycin

This study identifies bottromycin's mechanism of action as selectively trapping EF-Tu/glycyl-tRNA complex on the ribosome, inhibiting translation at glycine codons.

Inhibition of translation termination by Drosocin, an antimicrobial peptide from fruit flies

This study identifies Drosocin as a type II PrAMP that inhibits protein synthesis termination by stalling ribosomes at stop codons, distinct from apidaecin.

Structural basis for the context-specific action of classic peptidyl transferase inhibitors.

The paper presents high-resolution structures of ribosomal complexes with chloramphenicol and specific nascent peptides, revealing how amino acids in the nascent chain modulate drug affinity and explain context-specific inhibition of translation.

Structural basis for the tryptophan sensitivity of TnaC-mediated ribosome stalling

The paper investigates how the TnaC-ribosome complex recognizes and responds to L-Trp, revealing a mechanism involving cleavage rates and ligand binding.

Retapamulin-assisted ribosome profiling reveals the alternative bacterial proteome

The paper uses retapamulin-assisted ribosome profiling to identify alternative translation start sites in E. coli, revealing new proteins and regulatory roles.