Yury S. Polikanov

This study presents structural analysis of ribosome-nascent chain complexes to elucidate how macrolides inhibit bacterial translation in a context-specific manner.

This study identifies bottromycin's mechanism of action as selectively trapping EF-Tu/glycyl-tRNA complex on the ribosome, inhibiting translation at glycine codons.

The paper shows FaRel2, a toxSAS, specifically targets tRNAGly and tRNAThr by recognizing the acceptor stem, with structural differences explaining specificity variations among toxSASs.

The paper introduces CIMPLE, a rationally designed CRISPRi pooled library, to uncover antibiotic targets by improving genomic coverage of essential genes during drug exposure.

This study determines the structural basis of Cfr-mediated antimicrobial resistance and identifies mechanisms for evading it through ribosome structures with Cfr-modified 23S rRNA.

This paper investigates how thermorubin inhibits translation by causing ribosome stalling and affecting tRNA binding and translation elongation.

This study identifies Drosocin as a type II PrAMP that inhibits protein synthesis termination by stalling ribosomes at stop codons, distinct from apidaecin.

The paper reports structures of non-arrested ribosome nascent chain complexes, revealing new insights into ribosome function and the mechanism of the peptidyl transferase center.

The paper presents high-resolution structures of ribosomal complexes with chloramphenicol and specific nascent peptides, revealing how amino acids in the nascent chain modulate drug affinity and explain context-specific inhibition of translation.

The paper discovers a new LAP antibiotic, phazolicin, which inhibits bacterial ribosomes in a species-specific manner by blocking the exit tunnel.

This study reveals a guanosine-switch mechanism that regulates the opposing activities of bifunctional Rel enzymes through allosteric changes.