Author

E. J. Sontheimer

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 3 Biochemistry· 2 Immunology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 5 Organism:human· 4 Research Focus:gene regulatory network inference· 3 Organism:mouse· 3 Research Focus:cancer & tumor microenvironment· 2 Approach:genetic perturbation / CRISPR· 2 Research Focus:host-pathogen & immune response· 2 Assay:bulk RNA-seq· 1

Frequent Co-Authors

A. Mir· 2 shared J. K. Watts· 2 shared K. Ponnienselvan· 2 shared P. Liu· 2 shared R. Ibraheim· 2 shared S. A. Wolfe· 2 shared W. Xue· 2 shared A. Desai· 1 shared

Activity by Year

2018-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 21 mutual co-author links

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2 shared papersBioengineering

A. Mir

Mutual co-authors in cluster

Author profile →

6 papers

Recent·Most cited

Addressing the dNTP bottleneck restricting prime editing activity

K. Ponnienselvan, P. Liu, T. Nyalile, S. Oikemus, A. T. Joynt, K. Kelly, D. Guo, Z. Chen, J. M. Lee, C. A. Schiffer, C. P. Emerson, N. D. Lawson, J. K. Watts, E. J. Sontheimer, J. Luban, S. A. Wolfe

This study identifies mutations to improve prime editing efficiency in low-dNTP environments by enhancing MMLV-reverse transcriptase and reducing SAMHD1 activity.

bioRxiv · Oct 21, 2023Biochemistry

Epigenetic regulation of innate immune genes and enhanced interleukin-10 expression underlie chronic subclinical Plasmodium chabaudi infection

L. de Souza Silva, Y. A. H. Nguyen, B. G. Monks, C. S. Forconi, J. N. Crabtree, T. Rodriguez, N. D. P. Tamburro, E. J. Sontheimer, G. L. Horvath, Z. Abdullah, E. Latz, D. R. Caffrey, E. Kurt-Jones, R. T. Gazzinelli, K. A. Fitzgerald, D. T. Golenbock

This study identifies epigenetic regulation of innate immune genes and increased IL-10 expression as mechanisms underlying chronic subclinical Plasmodium chabaudi infection in mice.

bioRxiv · Feb 23, 2023 · PreprintImmunology

Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice

P. Liu, S. Liang, C. Zheng, E. Mintzer, Y. G. Zhao, K. Ponnienselvan, A. Mir, E. J. Sontheimer, G. Gao, T. R. Flotte, S. Wolfe, W. Xue

This paper improves prime editors for genome editing in adult mice, enabling pathogenic mutation correction and cancer modeling.

Nature Communications · Apr 9, 2021Peer-reviewedBioengineering

CRISPR-enhanced human adipocyte 'browning' as cell therapy for metabolic disease

E. Tsagkaraki, S. Nicoloro, T. De Souza, J. Solivan-Rivera, A. Desai, Y. Shen, M. Kelly, A. Guilherme, F. Henriques, R. Ibraheim, N. Amrani, K. Luk, S. Maitland, R. H. Friedline, L. Tauer, , M. P. Czech

This paper uses CRISPR to enhance browning of human adipocytes, improving metabolic health in mice without immunogenic Cas9 exposure.

bioRxiv · Oct 13, 2020Bioengineering

Tissue-specific Genome Editing in vivo by MicroRNA-repressible Anti-CRISPR Proteins

J. Lee, H. Mou, R. Ibraheim, S.-Q. Liang, W. Xue, E. J. Sontheimer

This paper develops a method to achieve tissue-specific genome editing in vivo using microRNA-repressible anti-CRISPR proteins to limit Cas9 activity to target tissues.

bioRxiv · May 8, 2019Bioengineering

Heavily and Fully Modified RNAs Guide Efficient SpyCas9-Mediated Genome Editing

A. Mir, J. F. Alterman, M. R. Hassler, A. J. Debacker, E. Hudgens, D. Echeverria, M. H. Brodsky, A. Khvorova, J. K. Watts, E. J. Sontheimer

This study identifies heavily and fully modified crRNA and tracrRNA that enhance Cas9-mediated genome editing efficiency and stability in human cells.

bioRxiv · Mar 28, 2018Biochemistry