G. F. Spaeth

MAPPING CHANGES OF MIRNA-MRNA NETWORKS IN LEISHMANIA-INFECTED MACROPHAGES PREDICTS REGULATORY MIRNA-TF LOOPS AS NOVEL TARGETS OF PARASITE IMMUNE SUBVERSION

Inhibition of the macrophage demethylase LSD1 reverses Leishmania amazonensis-induced transcriptomic changes and causes a decrease in parasite load

This study shows that inhibiting LSD1 in macrophages reduces Leishmania parasite load and reverses infection-induced transcriptomic changes.

Leishmania amazonensis controls macrophage-regulated cell death to establish chronic infection in vitro and in vivo

This study shows Leishmania amazonensis suppresses macrophage-regulated cell death pathways to promote chronic infection in vitro and in vivo.

The m6A reader IGF2BP2 directs immune-metabolic reprogramming in Leishmania amazonensis-infected macrophages

This study identifies IGF2BP2 as an m6A reader that stabilizes glycolytic transcripts in Leishmania-infected macrophages, promoting HIF-1-independent glycolysis and immune-metabolic reprogramming.

Leishmania allelic selection during experimental sand fly infection correlates with mutational signatures of oxidative DNA damage

This study identifies oxidative DNA damage signatures in Leishmania donovani during sand fly infection, linking them to allelic and haplotype selection through genomic analysis.

SILAKin: A novel high throughput SILAC and mass spectrometry-based assay to identify the substratome of kinases secreted by pathogens

SILAkin identifies kinase substrates using a combination of phosphatase treatment, pulse heating, IVKA, and SILAC-based MS, revealing 225 host substrates phosphorylated by pathogen-excreted kinases.

Experimental evolution reveals post-transcriptional regulation as a novel driver of Leishmania fitness gain

This study shows that post-transcriptional regulation, not just genome instability, drives fitness gain in Leishmania parasites during in vitro adaptation.

The low complexity regions in the C-terminus are essential for the subcellular localisation of Leishmania casein kinase 1 but not for its activity

This study identifies low complexity regions in the C-terminus of Leishmania CK1.2 as essential for its subcellular localization but not activity, revealing new insights into its role in parasite biology.

Dynamic High-Content Imaging Reveals Surface Exposure Of Virulent Leishmania Amastigotes In Infected Macrophages Undergoing Pyroptosis

This study shows that Leishmania-infected macrophages undergo pyroptosis, exposing amastigotes to the extracellular environment, which may aid parasite spread.

Leishmania targets the macrophage epigenome and dampens the NF-κB/NLRP3- mediated inflammatory response

This study shows Leishmania amazonensis modulates macrophage epigenetics to suppress NF-κB and NLRP3 inflammasome activation, aiding parasite survival.