J. M. Skotheim

Genome concentration limits cell growth and modulates proteome composition in Escherichia coli

The paper shows genome concentration limits transcription and affects proteome composition in E. coli, with effects detectable in nutrient-rich conditions.

Genome dilution by cell growth drives starvation-like proteome remodeling in mammalian and yeast cells

The paper shows that increasing cell size leads to proteome remodeling similar to starvation, driven by genome dilution in mammalian and yeast cells.

Cell size contributes to single-cell proteome variation

The paper shows that cell size significantly affects protein concentration variation in single-cell proteomics data.

Increasing cell size remodels the proteome and promotes senescence

The paper shows that increasing cell size remodels the proteome and promotes senescence, but polyploidization can prevent this by altering the DNA-to-ploidy ratio.

Localized phosphorylation of RNA Polymerase II by G1 cyclin-Cdk promotes cell cycle entry

The paper shows that G1 cyclin-Cdk phosphorylates RNA Polymerase II at SBF-regulated promoters to activate transcription and promote cell cycle entry.

Size-independent mRNA synthesis and chromatin-based partitioning mechanisms generate and maintain constant amounts of protein per cell

The paper identifies transcriptional and chromatin-based mechanisms that generate and maintain constant protein amounts per cell despite changes in cell size.

A G1 sizer mechanism coordinates growth and division in the mouse epidermis

The paper analyzes mouse epidermis time-lapse images to show that cell size homeostasis in vivo is governed by a G1 sizer mechanism, contrasting with the adder mechanism typically observed in vitro.

Multiple layers of phospho-regulation coordinate metabolism and the cell cycle in budding yeast

This study identifies cell cycle-dependent phosphorylation and dephosphorylation of metabolic enzymes in budding yeast, revealing mechanisms linking the cell cycle to metabolic regulation.

Constitutive expression of a fluorescent protein reports the size of live human cells

This paper introduces a fluorescent protein reporter to measure live human cell size, showing it is more robust than existing methods and useful for studying size-dependent cell cycle regulation.

Cell growth dilutes the cell cycle inhibitor Rb to trigger cell division

The paper shows that cell growth dilutes the cell cycle inhibitor Rb to drive cell division in human cells, establishing a molecular mechanism for cell size homeostasis.

Cyclin D-Cdk4,6 drives cell cycle progression via the retinoblastoma protein’s C-terminal helix

This study shows cyclin D-Cdk4,6 drives cell cycle progression by phosphorylating Rb's C-terminal helix, which is distinct from other cyclin-Cdk interactions and critical for tumor suppression.