J. Miles

Massively parallel mutant selection identifies genetic determinants of Pseudomonas aeruginosa colonization of Drosophila melanogaster

This study uses transposon sequencing to identify Pseudomonas aeruginosa genes critical for colonization of Drosophila melanogaster, revealing conserved and host-specific factors.

Evaluating Stored Platelet Shape Change Using Imaging Flow Cytometry

This paper develops an imaging flow cytometry method to quantify temperature-induced platelet shape change, showing a consistent transition from fusiform to circular shapes in mouse and human platelets.

Nonstructural protein 1 (nsp1) widespread RNA decay phenotype varies among Coronaviruses

This study compares the transcriptional targets and interactomes of four coronavirus nsp1 proteins, revealing virus-specific effects on host gene expression and protein interactions.

Black Dots: Microcontact-Printed, Reference-Free Traction Force Microscopy

This paper introduces a traction force microscopy technique called 'black dots' to measure cellular forces without constraining cell shape, finding that higher force correlates with increased spread area, circularity, and F-actin uniformity in platelets.

Enhanced lipogenesis through Pparγ helps cavefish adapt to food scarcity.

This study shows that cavefish enhance lipogenesis via upregulated Pparγ to store more fat, aiding adaptation to nutrient scarcity.

Immunogenomic profile of colorectal cancer response to immune checkpoint blockade

This study identifies immunogenomic features predicting response to immune checkpoint blockade in colorectal cancer, showing that high clonality and immune escape mechanisms are more important than mutational burden.

The KSHV ORF20 Protein Interacts With The Viral Processivity Factor ORF59 And Promote Viral Reactivation.

This study identifies ORF20 as a key regulator of KSHV lytic reactivation by interacting with ORF59 and promoting vDNA replication and virion production.

Expansion of Functional Regulatory T Cells Using Soluble RAGE Prevents Type 1 Diabetes

This study shows that soluble RAGE (sRAGE) expands functional regulatory T cells and prevents type 1 diabetes in mice and human T cells.

Hookworm-derived small molecule extracts suppress pathology in a mouse model of colitis and inhibit secretion of key inflammatory cytokines in primary human leukocytes

This study identifies anti-inflammatory small molecules from hookworms that reduce colitis pathology in mice and inhibit cytokine secretion in human leukocytes.