S. Nielsen

An ultrasound-guided biopsy technique for obtaining supraclavicular brown fat biopsies and preadipocytes

This paper describes an ultrasound-guided biopsy technique to collect supraclavicular brown fat and subcutaneous white fat from healthy individuals, and evaluates in vitro differentiation and gene expression of progenitor cells.

DNA targeting by compact Cas9d and its resurrected ancestor

This study reports cryo-EM structures of Cas9d, revealing its DNA targeting mechanism and demonstrating a compact variant with robust nuclease activity in mammalian cells.

Exercise Training Remodels Adipocyte-Specific Extracellular Matrix Proteins and Alters Innervation and Vascularization in Inguinal White Adipose Tissue (iWAT)

This study shows that exercise training remodels iWAT ECM, increases vascularization and innervation, and alters adipocyte subpopulations in male mice.

Cold-induced expression of a truncated Adenylyl Cyclase 3 acts as rheostat to brown fat function.

The paper identifies a cold-inducible truncated Adcy3 mRNA isoform, Adcy3-at, which acts as a rheostat to regulate brown fat function by limiting cAMP activity during obesity.

Purification of mitochondria from skeletal muscle tissue for transcriptomic analyses reveals localisation of nuclear-encoded non-coding RNAs

The paper develops a method to purify mitochondria from skeletal muscle tissue and identifies nuclear-encoded non-coding RNAs localized within mitochondria using transcriptomic analyses.

Polarized α-synuclein trafficking and transcytosis across Brain Endothelial Cells via Rab7-decorated carriers

This study identifies Rab7-decorated carriers mediating polarized transcytosis of monomeric α-synuclein across brain endothelial cells, suggesting Rab7 as a therapeutic target for Parkinson's disease.

Do male and female heterogamety really differ in expression regulation? Lack of global dosage balance in pygopodid geckos

The paper examines gene dosage balance in pygopodid geckos and finds no significant difference in expression regulation between male and female heterogamety.

Thermogenic genes are blunted whereas brown adipose tissue identity is preserved in human obesity

The paper identifies a reduction in thermogenic genes but preserved brown adipose tissue identity in human obesity through transcriptomic analysis of BAT and WAT.

Proteomics-based comparative mapping of the human brown and white adipocyte secretome reveals EPDR1 as a novel batokine

The paper compares the secretomes of human brown and white adipocytes using proteomics, identifying EPDR1 as a novel batokine involved in thermogenesis and energy consumption.

A long-non-coding RNA, LINC00473, confers the human adipose tissue thermogenic phenotype through enhanced cAMP responsiveness.

This study identifies LINC00473 as a key regulator of thermogenic adipocyte function, showing its role in enhancing cAMP responsiveness and mitochondrial activity.

The smallest intestine (TSI) - a low volume in vitro model of the small intestine with increased throughput

The paper develops a low-volume in vitro model of the small intestine (TSI) to study probiotic survival, showing that ileal microbiota significantly impacts probiotic survival.