A third dose of a single T cell epitope vaccine protects mice from SARS-CoV-2 infection without neutralizing antibodies by enhancing effector-memory and tissue-resident memory T cells.

bioRxiv · Dec 15, 2021 · PreprintImmunology

Ad26.COV2.S-elicited immunity protects against G614 spike variant SARS-CoV-2 infection in Syrian hamsters and does not enhance respiratory disease in challenged animals with breakthrough infection after sub-optimal vaccine dosing

J. E. M. van der Lubbe, S. K. Rosendahl Huber, A. Vijayan, L. Dekking, E. van Huizen, J. Vreugdenhil, Y. Choi, M. R. M. Baert, K. Feddes-de Boer, A. Izquierdo Gil, M. van Heerden, T. J. Dalebout, S. K. Myeni, M. Kikkert, E. J. Snijder, , F. Wegmann

This study shows that a single dose of Ad26.COV2.S vaccine protects Syrian hamsters from G614 SARS-CoV-2 infection and prevents VAERD even with suboptimal immunity.

bioRxiv · Jan 8, 2021 · PreprintImmunology

Immunogenicity and protective efficacy of one- and two-dose regimens of the Ad26.COV2.S COVID-19 vaccine candidate in adult and aged rhesus macaques

L. Solforosi, H. Kuipers, S. K. Rosendahl Huber, J. E. M. van der Lubbe, L. Dekking, D. N. Czapska-Casey, A. Izquierdo Gil, M. Baert, J. Drijver, J. Vaneman, E. van Huizen, Y. Choi, J. Vreugdenhil, S. Kroos, A. H. de Wilde, , R. C. Zahn

This study evaluates one- and two-dose regimens of the Ad26.COV2.S vaccine in rhesus macaques, showing higher antibody responses with two doses and protection against SARS-CoV-2 in aged animals.

bioRxiv · Nov 17, 2020Immunology

Ad26-vector based COVID-19 vaccine encoding a prefusion stabilized SARS-CoV-2 Spike immunogen induces potent humoral and cellular immune responses

R. Bos, L. Rutten, J. E. M. van der Lubbe, M. J. G. Bakkers, G. Hardenberg, F. Wegmann, D. Zuijdgeest, A. H. de Wilde, A. Koornneef, A. Verwilligen, D. van Manen, T. Kwaks, R. Vogels, T. J. Dalebout, S. K. Myeni, , H. Schuitemaker

This paper evaluates a prefusion-stabilized SARS-CoV-2 Spike protein vaccine candidate, Ad26.COV2.S, showing potent neutralizing and Th1-polarized immune responses in mice.

bioRxiv · Jul 30, 2020Immunology

SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology

N. Ogando, T. Dalebout, J. C. Zevenhoven-Dobbe, R. W. A. L. Limpens, Y. van der Meer, L. Caly, J. Druce, J. de Vries, M. Kikkert, M. Barcena, I. Sidorov, E. J. Snijder

This study compares SARS-CoV-2 and SARS-CoV replication in Vero E6 cells, finding higher viral RNA levels in SARS-CoV-2 but lower infectious progeny, and adaptive mutations in its spike protein.

bioRxiv · Apr 20, 2020Microbiology

The deubiquitinating activity of Middle East respiratory syndrome coronavirus papain-like protease delays the innate immune response and enhances virulence in a mouse model

R. Knaap, R. Fernandez-Delgado, T. J. Dalebout, N. Oreshkova, P. J. Bredenbeek, L. Enjuanes, I. Sola, E. J. Snijder, M. Kikkert

This study shows that disrupting the deubiquitinating activity of MERS-CoV PLpro enhances innate immune responses and reduces virulence in mice.

bioRxiv · Aug 29, 2019 · PreprintMicrobiology