Author

E. J. Snijder

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Microbiology· 11 Immunology· 3 Biochemistry· 1 Cell Biology· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 15 Research Focus:host-pathogen & immune response· 10 Research Focus:drug response / drug discovery· 4 Organism:human· 4 Assay:bulk RNA-seq· 3 Organism:mouse· 3 Research Focus:differential expression· 2 Assay:electronic health records· 2

Frequent Co-Authors

M. Barcena· 5 shared M. Kikkert· 5 shared A. H. de Wilde· 4 shared R. W. A. L. Limpens· 4 shared T. J. Dalebout· 4 shared A. J. Koster· 3 shared A. W. M. de Jong· 3 shared C. C. Posthuma· 3 shared

Activity by Year

2018-2022 · peak 8/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

5 shared papersMicrobiology

M. Barcena

Mutual co-authors in cluster

Author profile →

16 papers

Recent·Most cited

Honokiol inhibits SARS-CoV-2 replication in cell culture

C. Salgado-Benvindo, A. A. Leijs, M. Thaler, A. Tas, J. L. Arbiser, E. J. Snijder, M. J. van Hemert

This study shows honokiol inhibits SARS-CoV-2 replication in cell culture with EC50 of 7.8 μM, effective against variants like Omicron and other human coronaviruses.

bioRxiv · Jul 26, 2022 · PreprintMicrobiology

Impact of human airway epithelial cellular composition on SARS-CoV-2 infection biology

Y. Wang, M. Thaler, D. Ninaber, A. M. van der Does, N. S. S. Ogando, H. Beckert, C. Taube, C. Salgado Benvindo Da Silva, E. J. Snijder, P. J. Bredenbeek, P. Hiemstra, M. J. van Hemert

This study examines how the composition of human airway epithelial cells affects SARS-CoV-2 infection, finding that prolonged culture time and higher proportions of ciliated and goblet cells increase viral load.

bioRxiv · Jul 21, 2021 · PreprintCell Biology

6',6'-Difluoro-aristeromycin is a potent inhibitor of MERS-coronavirus replication

N. S. S. Ogando, J. Zevenhoven-Dobbe, D. Jarhad, S. K. Tripathi, H. W. Lee, L. S. Jeong, E. J. Snijder, C. C. Posthuma

This paper shows that 6',6'-difluoro-aristeromycin inhibits MERS-CoV replication but is cytotoxic and needs improvement for broader antiviral use.

bioRxiv · May 20, 2021 · PreprintMicrobiology

Ad26.COV2.S-elicited immunity protects against G614 spike variant SARS-CoV-2 infection in Syrian hamsters and does not enhance respiratory disease in challenged animals with breakthrough infection after sub-optimal vaccine dosing

J. E. M. van der Lubbe, S. K. Rosendahl Huber, A. Vijayan, L. Dekking, E. van Huizen, J. Vreugdenhil, Y. Choi, M. R. M. Baert, K. Feddes-de Boer, A. Izquierdo Gil, M. van Heerden, T. J. Dalebout, S. K. Myeni, M. Kikkert, E. J. Snijder, , F. Wegmann

This study shows that a single dose of Ad26.COV2.S vaccine protects Syrian hamsters from G614 SARS-CoV-2 infection and prevents VAERD even with suboptimal immunity.

bioRxiv · Jan 8, 2021 · PreprintImmunology

Immunogenicity and protective efficacy of one- and two-dose regimens of the Ad26.COV2.S COVID-19 vaccine candidate in adult and aged rhesus macaques

L. Solforosi, H. Kuipers, S. K. Rosendahl Huber, J. E. M. van der Lubbe, L. Dekking, D. N. Czapska-Casey, A. Izquierdo Gil, M. Baert, J. Drijver, J. Vaneman, E. van Huizen, Y. Choi, J. Vreugdenhil, S. Kroos, A. H. de Wilde, , R. C. Zahn

This study evaluates one- and two-dose regimens of the Ad26.COV2.S vaccine in rhesus macaques, showing higher antibody responses with two doses and protection against SARS-CoV-2 in aged animals.

bioRxiv · Nov 17, 2020Immunology

The enzymatic activity of the nsp14 exoribonuclease is critical for replication of Middle East respiratory syndrome-coronavirus

N. S. Ogando, J. Zevenhoven-Dobbe, C. C. Posthuma, E. J. Snijder

This study shows that the ExoN activity of MERS-CoV nsp14 is essential for viral RNA synthesis, unlike in MHV and SARS-CoV where it primarily enhances replication fidelity.

Journal of Virology · Nov 9, 2020Peer-reviewedMicrobiology

Ad26-vector based COVID-19 vaccine encoding a prefusion stabilized SARS-CoV-2 Spike immunogen induces potent humoral and cellular immune responses

R. Bos, L. Rutten, J. E. M. van der Lubbe, M. J. G. Bakkers, G. Hardenberg, F. Wegmann, D. Zuijdgeest, A. H. de Wilde, A. Koornneef, A. Verwilligen, D. van Manen, T. Kwaks, R. Vogels, T. J. Dalebout, S. K. Myeni, , H. Schuitemaker

This paper evaluates a prefusion-stabilized SARS-CoV-2 Spike protein vaccine candidate, Ad26.COV2.S, showing potent neutralizing and Th1-polarized immune responses in mice.

bioRxiv · Jul 30, 2020Immunology

A molecular pore spans the double membrane of the coronavirus replication organelle

G. Wolff, R. W. A. L. Limpens, J. C. Zevenhoven-Dobbe, U. Laugks, S. Zheng, A. W. M. de Jong, R. I. Koning, D. A. Agard, K. Grünewald, A. J. Koster, E. J. Snijder, M. Barcena

The paper identifies a molecular pore in coronavirus replication organelles that facilitates RNA export to the cytosol, suggesting it as a potential drug target.

bioRxiv · Jun 25, 2020Microbiology

Suramin inhibits SARS-CoV-2 infection in cell culture by interfering with early steps of the replication cycle

C. Salgado-Benvindo, M. Thaler, A. Tas, N. S. Ogando, P. J. Bredenbeek, D. Ninaber, Y. Wang, P. Hiemstra, E. J. Snijder, M. J. van Hemert

This study shows suramin inhibits SARS-CoV-2 replication in cell cultures by targeting early replication steps, with potential for clinical evaluation.

bioRxiv · May 6, 2020Microbiology

SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology

N. Ogando, T. Dalebout, J. C. Zevenhoven-Dobbe, R. W. A. L. Limpens, Y. van der Meer, L. Caly, J. Druce, J. de Vries, M. Kikkert, M. Barcena, I. Sidorov, E. J. Snijder

This study compares SARS-CoV-2 and SARS-CoV replication in Vero E6 cells, finding higher viral RNA levels in SARS-CoV-2 but lower infectious progeny, and adaptive mutations in its spike protein.

bioRxiv · Apr 20, 2020Microbiology

A unifying structural and functional model of the coronavirus replication organelle: tracking down RNA synthesis

E. J. Snijder, R. W. A. L. Limpens, A. H. de Wilde, A. W. M. de Jong, J. C. Zevenhoven-Dobbe, H. J. Maier, F. G. A. Faas, A. J. Koster, M. Barcena

This study identifies double-membrane vesicles (DMVs) as the central site for viral RNA synthesis in coronaviruses, providing a unifying model of the replication organelle.

bioRxiv · Mar 24, 2020Microbiology

Alpha-ketoamides as broad-spectrum inhibitors of coronavirus and enterovirus replication

L. Zhang, D. Lin, Y. Kusov, Y. Nian, Q. Ma, J. Wang, A. von Brunn, P. Leyssen, K. Lanko, J. Neyts, A. de Wilde, E. J. Snijder, H. Liu, R. Hilgenfeld

This study identifies alpha-ketoamides as broad-spectrum inhibitors effective against coronavirus and enterovirus proteases, with compounds showing low-micromolar activity in cell cultures.

bioRxiv · Feb 10, 2020 · PreprintBiochemistry

The deubiquitinating activity of Middle East respiratory syndrome coronavirus papain-like protease delays the innate immune response and enhances virulence in a mouse model

R. Knaap, R. Fernandez-Delgado, T. J. Dalebout, N. Oreshkova, P. J. Bredenbeek, L. Enjuanes, I. Sola, E. J. Snijder, M. Kikkert

This study shows that disrupting the deubiquitinating activity of MERS-CoV PLpro enhances innate immune responses and reduces virulence in mice.

bioRxiv · Aug 29, 2019 · PreprintMicrobiology

Mind the gap: micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellae

G. Wolff, R. W. A. L. Limpens, S. Zheng, E. J. Snijder, D. Agard, A. J. Koster, M. Barcena

This paper introduces micro-expansion joints to reduce lamella bending during cryo-FIB-milling, improving structural studies of biological samples.

bioRxiv · May 31, 2019Microbiology

Inhibition of arterivirus RNA synthesis by cyclophilin inhibitors is counteracted by mutations in replicase transmembrane subunits

A. H. De Wilde, L. Boomaars-van der Zanden, A. W. M. de Jong, M. Barcena, E. J. Snijder, C. C. Posthuma

This study identifies adaptive mutations in nsp2 and nsp5 of equine arteritis virus that confer resistance to cyclophilin inhibitors, revealing their role in the mechanism of action of these antiviral compounds.

bioRxiv · Mar 24, 2019Microbiology

The transcriptional and translational landscape of equine torovirus

H. Stewart, K. Brown, A. M. Dinan, N. Irigoyen, E. J. Snijder, A. Firth

This study analyzes the transcription and translation of equine torovirus using RNA-seq and Ribo-seq, identifying novel open reading frames and subgenomic RNA synthesis mechanisms.

bioRxiv · Apr 7, 2018Microbiology