R. T. Hay

Mechanism of degrader-targeted protein ubiquitinability

The paper solves cryo-EM structures of a degrader-mediated complex to reveal structural insights into target protein ubiquitination mechanisms.

PML mutants resistant to arsenic induced degradation fail to generate the appropriate SUMO and ubiquitin signals required for RNF4 and p97 recruitment

The paper investigates arsenic resistance in PML mutants by analyzing SUMO and ubiquitin signaling and their impact on RNF4 and p97 recruitment.

Negative modulation of macroautophagy by HERPUD1 is counteracted by an increased ER-lysosomal network with impact in drug-induced stress cell survival

The paper identifies HERPUD1 as a negative regulator of macroautophagy, showing that its stabilization promotes ER-lysosomal network expansion and enhances cell survival under stress.

Identification of SUMO targets required to maintain human stem cells in the pluripotent state.

This study identifies SUMO targets critical for maintaining human pluripotency by using a SUMO inhibitor and SUMOylation site mapping in hiPSCs.

Ubiquitin transfer by a RING E3 ligase occurs from a closed E2~Ub conformation

This study uses smFRET to show that ubiquitin transfer by RING E3 ligases occurs from a closed E2~Ub conformation.

SUMOylation targets shugoshin to stabilize sister kinetochore biorientation

This study identifies SUMOylation as a mechanism that stabilizes sister kinetochore biorientation by modulating Aurora B kinase and PP2A interactions.

Functional 3D architecture in an intrinsically disordered E3 ligase domain facilitates ubiquitin transfer

This study reveals how the intrinsically disordered substrate-recognition domain of RNF4 adopts a defined 3D architecture to facilitate SUMO binding and ubiquitin transfer.

Sumoylation regulates central spindle protein dynamics during chromosome segregation in oocyte

The paper shows SUMO regulates BUB-1 and CLS-2CLASP localization during anaphase I in C. elegans oocytes, ensuring proper chromosome segregation.

Characterisation Of The Biflavonoid Hinokiflavone As A Pre-mRNA Splicing Modulator That Inhibits SENP

This study identifies hinokiflavone as a splicing inhibitor that modulates alternative splicing by inhibiting SUMO protease SENP1 and altering SUMOylation of splicing factors.