S. Uemura

Combined and differential roles of ADD domains of DNMT3A and DNMT3L on DNA methylation landscapes in mouse germ cells

This study examines the roles of ADD domains in DNMT3A and DNMT3L in DNA methylation in mouse germ cells, finding that their combined loss leads to severe methylation defects.

Polycomb repressive complex 1.1 coordinates homeostatic and emergency myelopoiesis

This study identifies PRC1.1 as a regulator of homeostatic and emergency myelopoiesis, showing its role in balancing myeloid differentiation and preventing transformation.

N-terminal region of Drosophila melanogaster Argonaute2 forms amyloid-like aggregates

This study shows that the N-terminal region of Drosophila melanogaster Argonaute2 forms amyloid-like aggregates, suggesting it functions as a prion-like domain.

Quantitative live-cell imaging of secretion activity reveals dynamic immune responses

This paper introduces qLCI-S, a method for quantitative live-cell imaging of secretion activity, enabling single-cell analysis of cytokine secretion and transcriptome profiling of rare cell populations.

Accumulation of TERT in Mitochondria Shows Two Opposing Effects on Apoptosis

This study shows that mitochondrial TERT has opposing effects on apoptosis: it initiates cell death early but delays it later, resolving a controversy about its role.

Implementation of single molecule FRET for visualizing intramolecular movement in CRISPR-Cas9

This paper describes methods to visualize flexible inter-domain movements of Cas9 using single molecule FRET and discusses necessary complementary experiments for accurate analysis.

Virtual-freezing fluorescence imaging flow cytometry

This paper introduces a virtual-freezing fluorescence imaging flow cytometry method that enhances throughput without sacrificing sensitivity and spatial resolution, enabling high-dimensional analysis and classification.

Real-time observation of flexible domain movements in Cas9

This study uses single-molecule FRET to observe dynamic domain movements in Cas9, showing flexibility enables DNA cleavage and sgRNA assembly.