O. Nureki

Structural insights into the agonist selectivity and structure-based engineering of the adenosine A3 receptor

The paper identifies a novel A3R-selective ligand and reveals structural insights into A3R activation, enabling structure-guided engineering of m6A-insensitive A3R for drug discovery.

Molecular basis of ligand promiscuity, structural mimicry, and atypical dimerization in the chemokine receptors

This study investigates ligand promiscuity and signaling bias in chemokine receptors by analyzing binding modes and determining cryo-EM structures of receptor-ligand complexes.

cryo-EM structure of a blue-shifted channelrhodopsin from Klebsormidium nitens.

The paper reports the cryo-EM structure of a blue-shifted channelrhodopsin from Klebsormidium nitens and develops mutants with blue-shifted action spectra.

Structure of a lasso peptide bound ETB receptor provides insights into the mechanism of GPCR inverse agonism

The paper reports cryo-EM structures of the ETB receptor bound to the lasso peptide RES-701-3, revealing its inverse agonist mechanism through hydrophobic interactions and conformational stabilization.

Structure and dynamics of the RF-amide QRFP receptor GPR103

This study reports the cryo-EM structure of the QRFP-GPR103-Gq complex, revealing unique binding mechanisms and insights into RF-amide peptide recognition.

Structural basis of anticancer drug recognition and amino acid transport by LAT1

This study reports six LAT1 structures bound with ligands, revealing substrate recognition and inhibition mechanisms, including the role of JPH203 as an anticancer drug.

Optimizing Cryo-EM Structural Analysis of Gi-coupling Receptors via Engineered Gt and Nb35 Application

The paper uses engineered G protein eGt with Nb35 to improve cryo-EM structure determination of GPCR-Gi complexes, achieving better map quality and reduced anisotropy.

Structure of full-length ERGIC-53 in complex with MCFD2 for cargo transport

This study determines the cryo-EM structure of full-length ERGIC-53 complexed with MCFD2, revealing its tetrameric architecture and mechanism of cargo transport.

Molecular insights into atypical modes of beta-arrestin interaction with seven transmembrane receptors

This study presents cryo-EM structures and experimental data revealing how beta-arrestins interact with GPCRs, particularly those with phosphorylation in the third intracellular loop and intrinsically biased receptors.

Structure of the human Bre1 complex bound to the nucleosome

The paper determines the cryo-EM structure of the human Bre1 complex bound to the nucleosome, revealing how it catalyzes H2B monoubiquitination and its potential role in cancer.

Cure of congenital purpura fulminans via expression of engineered protein C through neonatal genome editing in mice

The paper demonstrates that ectopic expression of an engineered protein C via neonatal genome editing cures congenital protein C deficiency in mice.

Structural basis for lysophosphatidylserine recognition by GPR34

This study presents cryo-EM structures of GPR34-Gi complex bound with LysoPS analogs, revealing ligand recognition mechanisms and identifying 2-acyl LysoPS as the physiological ligand.

Cryo-EM structure of the endothelin-1-ETB-Gi complex

The paper reports a cryo-EM structure of the endothelin-1-ETB-Gi complex, revealing structural changes upon G-protein activation and unique binding characteristics.

Energy transfer in ubiquitous rhodopsin pumps with xanthophyll antennas

The paper detects energy transfer from hydroxylated carotenoids to rhodopsin proton pumps in marine and freshwater bacteria.

Mechanistic insights into intramembrane proteolysis by E. coli site-2 protease homolog RseP

This study elucidates the mechanism of intramembrane proteolysis by E. coli RseP through crystal structures and mutational analysis, revealing a gating mechanism for substrate entry.

Structural basis of the regulation of normal and oncogenic methylation of nucleosomal histone H3 Lys36 by NSD2

This study determines the cryo-EM structure of NSD2 bound to a nucleosome and identifies oncogenic mutations that increase its catalytic activity by altering the autoinhibitory loop conformation.

Structural basis for channel conduction in the pump-like channelrhodopsin ChRmine

The paper presents the cryo-EM structure of ChRmine, revealing unique architectural features and provides insights into its conduction mechanism and potential for optogenetic applications.

Cryo-EM structure of the MgtE Mg2+ channel pore domain in Mg2+-free conditions reveals cytoplasmic pore opening

The paper determines the cryo-EM structure of MgtE in Mg2+-free conditions, revealing cytoplasmic pore opening and kink motions in TM2 and TM5 helices.

Crystal structure of schizorhodopsin reveals mechanism of inward proton pumping

The paper reports the crystal structure of schizorhodopsin, revealing its mechanism of inward proton pumping and differences from xenorhodopsins.

Structural basis for no spectral shift of heliorhodopsin by counterion mutation

The paper presents the crystal structure of a HeR mutant with an identical absorption spectrum to wild-type, revealing a unique spectral shift mechanism through counterion and serine cluster interactions.

Structural insights into the mechanism of rhodopsin phosphodiesterase

This study presents the structural and functional analysis of Rh-PDE from Salpingoeca rosetta, revealing its photoactivation mechanism and expanding the definition of rhodopsins.

A single CRISPR base editor to induce simultaneous C-to-T and A-to-G mutations

This paper introduces Target-ACE, a CRISPR base editor that induces both C-to-T and A-to-G mutations, showing higher efficiency than using separate editors.

Cryo-EM structures capturing the entire transport cycle of the P4-ATPase flippase

The paper reports cryo-EM structures of six intermediates in the ATP8A1-CDC50a flippase cycle, revealing the mechanism of phospholipid translocation.

Cryo-EM structure of the human L-type amino acid transporter 1 in complex with glycoprotein CD98hc

This paper reports the cryo-EM structure of the human LAT1-CD98hc heterodimer, revealing its architecture and interactions, and maps disease mutations to explain congenital disorders.

Crystal structure of human endothelin ETB receptor in complex with peptide inverse agonist IRL2500

The paper reports the crystal structure of the human ETB receptor complexed with the inverse agonist IRL2500, revealing its binding mechanism and ETB-selectivity.

Free energy landscape for the entire transport cycle of triose-phosphate/phosphate translocator

This study uses molecular dynamics simulations to reveal the free energy landscape and mechanism of the triose-phosphate/phosphate translocator's transport cycle.

Real-time observation of flexible domain movements in Cas9

This study uses single-molecule FRET to observe dynamic domain movements in Cas9, showing flexibility enables DNA cleavage and sgRNA assembly.

Structure of the triose-phosphate/phosphate translocator reveals the basis of substrate specificity

This study determines the crystal structures of TPT bound to substrates, revealing the molecular basis of its strict 1:1 exchange mechanism.

Atomic resolution structure of serine protease proteinase K at ambient temperature

The paper reports an atomic resolution structure of proteinase K at ambient temperature using serial femtosecond crystallography, revealing hydrogen atoms, water molecules, and side-chain conformations.