Author

O. Ohara

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Biochemistry· 2 Immunology· 2 Bioengineering· 1 Biophysics· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 5 Organism:human· 4 Assay:proteomics· 4 Research Focus:cell type annotation· 3 Research Focus:cell signaling & trafficking· 2 Research Focus:drug response / drug discovery· 2 Research Focus:host-pathogen & immune response· 2 Organism:mouse· 2

Frequent Co-Authors

T. Watanabe· 3 shared D. Nakajima· 2 shared M. Ishikawa· 2 shared R. Konno· 2 shared Y. Kawashima· 2 shared A. Kamata· 1 shared E. Hiejima· 1 shared F. Yokoyama· 1 shared

Activity by Year

2021-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 13 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

3 shared papersImmunology

T. Watanabe

Mutual co-authors in cluster

Author profile →

6 papers

Recent·Most cited

Thin-diaPASEF: diaPASEF for maximizing proteome coverage in single-shot proteomics

R. Konno, M. Ishikawa, D. Nakajima, K. Inukai, O. Ohara, Y. Kawashima

The paper develops Thin-diaPASEF, an optimized diaPASEF method for single-shot proteomics, achieving high protein identification and coverage in HEK cells and multi-sample analyses.

bioRxiv · Apr 26, 2024Biochemistry

Universal pretreatment development for low-input proteomics using Lauryl Maltose Neopentyl Glycol

R. Konno, M. Ishikawa, D. Nakajima, Y. Endo, O. Ohara, Y. Kawashima

The paper develops a low-input proteomics method called LASP to improve peptide recovery and reduce MS contamination, achieving 1,175 proteins identified per single cell.

Molecular & Cellular Proteomics · Apr 1, 2024Peer-reviewedBiochemistry

Development of a new genotype-phenotype linked antibody screening system

T. Watanabe, H. Hata, Y. Mochizuki, F. Yokoyama, T. Hasegawa, N. Kumar, T. Kurosaki, O. Ohara, H. Fukuyama

This paper develops a new antibody screening system using a Golden Gate-based dual-expression vector to rapidly isolate high-affinity cross-reactive antibodies from immunized mice.

bioRxiv · Mar 6, 2024Bioengineering

Quantitative live-cell imaging of secretion activity reveals dynamic immune responses

M. Yamagishi, K. Miyata, T. Kamatani, H. Kabata, R. Baba, Y. Tanaka, N. Suzuki, M. Matsuzaka, Y. Motomura, T. Kiniwa, S. Koga, K. Goda, O. Ohara, T. Funatsu, K. Fukunaga, , Y. Shirasaki

This paper introduces qLCI-S, a method for quantitative live-cell imaging of secretion activity, enabling single-cell analysis of cytokine secretion and transcriptome profiling of rare cell populations.

bioRxiv · Feb 9, 2022Biophysics

Aberrant localization of CDC42 C-terminal variants to the Golgi apparatus drives pyrin inflammasome-dependent autoinflammation

M. Nishitani-Isa, K. Mukai, Y. Honda, H. Nihira, H. Shibata, T. Tanaka, K. Kodama, E. Hiejima, K. Izawa, Y. Kawasaki, M. Osawa, Y. Katata, S. Onodera, T. Watanabe, S. Kure, , T. Yasumi

This study identifies that CDC42 C-terminal variants cause aberrant Golgi localization, leading to pyrin inflammasome activation and autoinflammation.

bioRxiv · Sep 3, 2021Immunology

Inhibition of immunoglobulin class-switching prevents pemphigus onset in desmoglein 3-specific B cell receptor knock-in mouse

H. Nomura, N. Wada, H. Takahashi, Y. Kase, J. Yamagami, S. Egami, H. Iriki, M. Mukai, A. Kamata, H. Ito, H. Fujii, T. Ishikura, H. Koseki, T. Watanabe, T. Yamada, , M. Amagai

This study shows that inhibiting immunoglobulin class-switching prevents pemphigus onset in mice, with FcγRIIB signaling attenuation contributing to disease.

bioRxiv · May 23, 2021 · PreprintImmunology