T. Pham

Label-Free Melanoma Phenotype Classification Using Artificial Intelligence-Based Morphological Profiling

This paper develops an AI-based classifier to classify melanoma cell phenotypes using morphology, validated by scRNA-Seq, showing morphology correlates with phenotypic states.

Discovery and engineering of the antibody response against a prominent skin commensal

The paper identifies an antibody response against Staphylococcus epidermidis and shows how it can be engineered for therapeutic vaccination.

Ultrasensitive and multiplexed protein imaging with clickable and cleavable fluorophores

This paper develops a highly sensitive and multiplexed in situ protein profiling method using click chemistry and cleavable fluorophores, enabling the quantification of 28 proteins in FFPE tissue and classification of over 820,000 cells.

Single-cell T cell receptor sequencing of paired tissue and blood samples reveals clonal expansion of CD8+ effector T cells in patients with calcific aortic valve disease

The paper uses single-cell T cell receptor sequencing to analyze paired tissue and blood samples from CAVD patients, identifying clonal expansion of CD8+ effector T cells and potential epitope targets in the valve.

Intracellular Proteomics and Extracellular Vesiculomics as a Metric of Disease Recapitulation in 3D Bioprinted Aortic Valve Arrays

This study compares 3D-bioprinted and 2D models of calcific aortic valve disease using proteomics and vesiculomics to assess disease recapitulation.

Endothelial cells secrete small extracellular vesicles bidirectionally containing distinct cargo to uniquely reprogram vascular cells in the circulation and vessel wall

This study shows endothelial cells release distinct extracellular vesicles to reprogram monocytes and smooth muscle cells in circulation and vessel wall.

The RAM signaling pathway links morphology, thermotolerance, and CO2 tolerance in the global fungal pathogen Cryptococcus neoformans

This study identifies the RAM signaling pathway's role in CO2 and thermotolerance in Cryptococcus neoformans and discovers novel regulators of virulence.

Measurement of Cervical Neuronal Activity during Stress Challenge Using Novel Flexible Adhesive Surface Electrodes

This paper introduces a novel flexible adhesive electrode array for non-invasive cervical nerve activity monitoring and demonstrates its use in detecting stress-induced neuronal changes.

DectiSomes: Glycan Targeting of Liposomal Drugs Improves the Treatment of Disseminated Candidiasis

This paper demonstrates that glycan-targeted liposomal antifungal drugs, DectiSomes, significantly reduce fungal burden and improve survival in a mouse model of disseminated candidiasis.

SARS-CoV-2 spike-glycoprotein processing at S1/S2 and S2' and shedding of the ACE2 viral receptor: roles of Furin and TMPRSS2 and implications for viral infectivity and cell-to-cell fusion

This study investigates the roles of Furin and TMPRSS2 in SARS-CoV-2 spike protein processing and viral entry, showing their synergistic effects and suggesting combined inhibitors as antivirals.

Human INCL fibroblasts display abnormal mitochondrial and lysosomal networks and heightened susceptibility to ROS-induced cell death

A novel drug-combination screen in zebrafish identifies epigenetic small molecule candidates for Duchenne muscular dystrophy

The paper identifies two epigenetic small molecules, oxamflatin and salermide, that improve skeletal muscle structure in zebrafish models of Duchenne muscular dystrophy through a drug-combination screen.

Characterization of GRK5 as a novel regulator of rhabdomyosarcoma tumor cell growth and self-renewal

This study identifies GRK5 as a novel regulator of rhabdomyosarcoma growth and self-renewal, showing its kinase-independent role in promoting tumor progression and suggesting it as a therapeutic target.

Dynamic centriolar relocalization of Polo kinase and Centrobin in early mitosis primes centrosomeasymmetry in fly neural stem cells

The paper shows Polo kinase and Centrobin relocalize from mother to daughter centrioles in fly neural stem cells during mitosis, establishing centrosome asymmetry that primes biased MTOC activity.