Wei Wu

High-throughput mapping of single-cell molecular and projection architecture of neurons by retrograde barcoded labelling

This paper develops a method to map single-cell neuronal projections and transcriptomes, revealing molecular heterogeneity in projection-defined neurons.

RNA degradation analysis reveals ribosome dynamics in complex microbiome samples

The paper analyzes RNA degradation in microbiome samples to study ribosome dynamics and species-specific responses to stress and drugs.

Multi-omics integrated analysis reveals a specific phenotype of CD8+ T cell may contribute to immunothromosis via Th17 response in severe and critical COVID-19

The paper identifies a specific CD8+ T cell phenotype in severe COVID-19 linked to Th17 response and immunothrombosis using multi-omics data.

Single-molecule, full-length transcript isoform sequencing reveals disease mutation-associated RNA isoforms in cardiomyocytes

The paper develops a pipeline to identify and quantify full-length RNA isoforms, revealing disease-associated isoforms in cardiomyocytes with RBM20 mutations.

Dissecting chronic myeloid leukaemia overlapping transcriptome with TIF-Seq2

The paper introduces TIF-Seq2, a method to sequence RNA 5 and 3 ends at single-nucleotide resolution, improving transcriptome annotation and identifying overlapping transcripts in chronic myeloid leukaemia.

High-fidelity base editor with no detectable genome-wide off-target effects

The paper identifies base editor variants with reduced off-target effects on both DNA and RNA.

Chromatin-sensitive cryptic promoters encode alternative protein isoforms in yeast.

The paper identifies chromatin-sensitive cryptic promoters in yeast that generate alternative protein isoforms through truncated mRNAs.

Base editing generates substantial off-target single nucleotide variants

This study compares off-target single nucleotide variants (SNVs) from CRISPR-Cas9 and base editing (BE3) in mouse embryos using the GOTI method, finding BE3 causes significantly more SNVs.