Vicent Pelechano

This study evaluates the trade-off between spatial resolution and proteome depth in spatial proteomics using SLACS, showing that few-cell sampling maintains proteomic information while increasing spatial specificity.

This study identifies cancer cell-state transitions using multimodal single-cell data by analyzing discordant mRNA and protein levels, revealing molecular drivers and prognostic signatures across multiple cancer types.

This paper introduces s5PSeq, a streamlined protocol for rapid antimicrobial sensitivity testing using 5P mRNA degradation intermediates, demonstrating its clinical utility in identifying resistant Clostridioides difficile isolates.

The paper shows that ribosomes modulate transcriptome abundance through frameshifts and out-of-frame mRNA decay in yeast under poor nutritional conditions, with conservation across species.

The paper shows that XRN1-mediated RNA decay provides nucleotides for alphavirus replication by recycling monophosphate nucleotides through the salvage pathway.

The paper analyzes RNA degradation in microbiome samples to study ribosome dynamics and species-specific responses to stress and drugs.

The paper shows the exosome degrades chromatin-associated RNAs and helps maintain chromatin homeostasis by regulating repetitive sequences and centromere organization.

The paper shows codon optimality affects mRNA stability across human tissues, with energy metabolism and age modulating this effect.

This study shows that Bud27 is required for RSC chromatin association and proper nucleosome positioning, leading to altered polyadenylation site usage in yeast.

The paper shows that Not1 and Not4 oppositely regulate mRNA solubility, affecting co-translational dynamics in yeast.

The paper identifies mRNA stability regulation as a factor modulating transcriptional memory in yeast, showing it enhances gene expression remodelling in primed cells.

GEMLI predicts cell lineages from scRNA-seq data without lineage tracing, identifying heritable gene expression and cell fate decisions in various contexts.

This study provides guidelines for accurate SARS-CoV-2 genotyping using amplicon-based sequencing, showing that at least 1000 viral genomes are needed to reliably detect variants at 10% frequency.

The paper introduces HT-5Pseq, a method to study degradation-associated ribosome stalls, finding that Arginine before stop codons is enriched in these stalls, while TAA stop codons are more common in stable mRNAs.

The paper introduces fivepseq, a tool for analyzing 5P degradome data, revealing insights into ribosome dynamics and gene-specific features in yeast and Arabidopsis.

This study identifies Rpb4 and Puf3 as regulators of mRNA stability and imprinting in yeast through genome-wide analysis and functional interactions.

The paper introduces TIF-Seq2, a method to sequence RNA 5 and 3 ends at single-nucleotide resolution, improving transcriptome annotation and identifying overlapping transcripts in chronic myeloid leukaemia.

The paper uses TIF-seq to identify TSS/PAS pairs in Arabidopsis, revealing widespread promoter-proximal transcriptional termination through sppRNAs.

The paper identifies chromatin-sensitive cryptic promoters in yeast that generate alternative protein isoforms through truncated mRNAs.

The paper investigates how RNAPII transcription represses intragenic promoters in plants by altering chromatin signatures and requiring the FACT complex.

The paper investigates how RNA-binding proteins (RBPs) change in response to viral infection and identifies RBPs crucial for viral replication.