Abhishek Mazumder

Unraveling single-molecule reactions via multiplexed in-situ DNA sequencing

This paper introduces SPIN-Seq, a method that links DNA sequence with functional properties at the single-molecule level, revealing sequence-dependent transcription factor interactions and RNA polymerase behavior.

Heterotypic Protein Interactions Modulate the Condensate Dynamics and Aggregation of α-Synuclein

This study investigates how heterotypic protein interactions affect α-synuclein condensate dynamics and aggregation, showing that multivalent electrostatic interactions drive co-condensation and promote amyloid fibril formation.

Closing and opening of the RNA polymerase trigger loop

The paper uses single-molecule fluorescence resonance energy transfer to study RNA polymerase trigger loop dynamics, showing it closes and opens on the millisecond timescale and functions as a checkpoint for NTP identity and inhibitor target.

One-dimensional sliding assists σ70-dependent promoter binding by Escherichia coli RNA polymerase

This study uses single-molecule imaging to show Escherichia coli RNA polymerase σ70 holoenzyme performs one-dimensional sliding on DNA during promoter search, even at high RNAP concentrations.

Structural basis of transcription inhibition by fidaxomicin (lipiarmycin A3)

This paper reports the cryo-EM structure of Mycobacterium tuberculosis RNAP holoenzyme bound to fidaxomicin, revealing its unique binding site and mechanism of transcription inhibition.