Manuela Melchionda

The paper shows that RA induces a transient co-expression state of PAX6 and FOXA2 in neural tube organoids, leading to two opposing cell fates that determine cell-type proportions through feedback signaling.

The paper develops a method to engineer synthetic cis-regulatory elements that control gene expression in response to signals, showing they can be designed using quantitative rules.

The paper compares protein dynamics in mouse and human neural progenitors, finding that human NPs have longer protein half-lives and lower proteasomal activity, which influences developmental tempo.

The paper shows how floorplate organiser forms autonomously in neural tube organoids through BMP-mediated cluster competition and FOXA2 dynamics.

The paper identifies a temporal patterning program in vertebrate neurons controlled by transcription factors and the TGFβ pathway, showing its role in generating neuronal diversity.

The paper shows that slower human embryonic development compared to mice is due to higher protein stability and longer cell cycle duration in human cells.

scRNA-seq of 21465 mouse neural tube cells (e9.5-e13.5) maps spinal progenitor and neuron types, validates new subtype markers, and reveals temporally staged transcription-factor modules.

The paper uses single cell transcriptomics to study Olig2 and Hes regulatory dynamics during motor neuron differentiation, revealing Olig2's role in repressing Hes5 and coordinating differentiation timing.