Kristian Honnens de Lichtenberg

Suspension culture drives metabolic rewiring in human pluripotent stem cells

This study compares 2D and 3D suspension cultures of human pluripotent stem cells, finding metabolic rewiring in 3D cultures with increased glycolysis and altered TCA cycle carbon partitioning.

Netrin-1 drives cell-type-specific plasticity of human dopaminergic neurons during circuit integration in a Parkinsonian model

This study identifies Netrin-1 as a DA-specific cue that enhances graft integration and functional recovery in Parkinsonian models without off-target effects.

Efficient Generation of Expandable Dorsal Forebrain Neural Rosette Stem Cell Lines

This study presents a method to generate pure and expandable neural rosette stem cell lines without manual isolation, demonstrating their differentiation potential and scalability for clinical applications.

Extensive NEUROG3 occupancy in the human pancreatic endocrine gene regulatory network

This study maps NEUROG3 occupancy in human pancreatic endocrine progenitors, identifying 1268 target genes and linking NEUROG3 to islet cell development and type 2 diabetes risk variants.

TGF-β modulates cell fate in human ES cell-derived foregut endoderm by inhibiting multiple endogenous signaling pathways

This study identifies how TGF-β1 modulates cell fate in human ES cell-derived foregut endoderm by inhibiting multiple signaling pathways, promoting pancreas fate over liver.

Jag1 modulates an oscillatory Dll1-Notch-Hes1 signaling module to coordinate growth and fate of pancreatic progenitors

This study shows Jag1 modulates oscillatory Dll1-Notch-Hes1 signaling in pancreatic progenitors to coordinate their growth and fate, with Jag1 also specifying most bipotent progenitors.

Notch Controls Multiple Pancreatic Cell Fate Regulators Through Direct Hes1-mediated Repression

This study identifies direct Hes1 target genes in pancreatic progenitor cells, revealing Notch signaling's role in repressing tip cell fate through repression of key regulators like Ptf1a and Gata4.

Genome-Wide Identification of HES1 Target Genes Uncover Novel Roles for HES1 in Pancreatic Development

The paper identifies HES1 target genes using ChIP-seq and RNA-seq in hESC lines, revealing HES1's role in pancreatic development through Notch pathway regulation and suppression of intestinal gene programs.