Roshan Sharma

The paper introduces mtDIVE, a random mtDNA mutagenesis platform, to study genotype-phenotype associations in metabolic contexts and lineages, revealing lineage-specific effects and mutation-specific impacts.

This study identifies an IL-1β/IL-6 signaling circuit in the tumor microenvironment that drives prostate cancer progression from pre-invasive to invasive stages.

The paper identifies MDF, a repair scar linked to POLQ-driven error-prone repair, as a marker of immune-responsive HRD pancreatic cancer with increased neoantigens and favorable clinical outcomes.

The paper analyzes transcriptomic changes in metastatic pancreatic cancer using a patient's autopsy data to identify site-specific gene programs and clonal phylogeny.

The paper presents a single-nucleus RNA sequencing atlas of Aedes aegypti mosquitoes, identifying novel cell types and sexual dimorphism in gene expression across tissues.

The paper identifies L1CAM-driven planar cell polarity signaling as a mechanism promoting SOX2+ metastatic progenitors in lung adenocarcinoma.

This study identifies an epigenetically distinct HSC subset, Kitlo, that supports better thymic and T-cell reconstitution in mice and humans.

This paper develops a method to engineer specific mtDNA deletions in human cells using end-joining machinery and targeted endonucleases, revealing a 75% heteroplasmy threshold for mitochondrial dysfunction.

The paper uses single-cell RNA and ATAC-seq to study bidirectional reprogramming between embryonic states, revealing differences in lineage plasticity.

The paper maps the developmental trajectories of endoderm cells in the mouse embryo to reveal lineage relationships and spatial organization during gut formation.

MAGIC improves single-cell RNA-seq data by imputing missing values and revealing gene-gene interactions through a diffusion-based method.

The paper develops methods to infer dynamic gene regulatory network rewiring during EMT from single-cell data and validates their predictive power for drug effects.