Takayuki Uchihashi

Silicon exaptation discovers antimicrobial peptides

The paper uses machine learning to discover antimicrobial peptides through an unintended functional property revealed by the algorithm.

Metastable Dimeric Intermediate Drives Native-like Spidroin Fibrillogenesis, while Dehydration Promotes Cross-β Transition

This paper identifies a metastable dimeric intermediate essential for forming native-like spidroin fibrils, but shows dehydration causes irreversible transition to amyloid-like cross-β structures.

High-speed atomic force-Raman microscopy

This paper introduces high-speed atomic force-Raman microscopy (HS-AFRM) to combine structural and chemical analysis, enhancing the understanding of biomolecular dynamics.

Dynamic organizational strategies of multidomain glycosyltransferases revealed by high-speed AFM and solution biophysics

The paper uses high-speed AFM and biophysical methods to study dynamic conformational changes in multidomain glycosyltransferases, revealing diverse organizational strategies.

A single conserved residue in FlhA couples export gate activation to substrate specificity switching

This study identifies a conserved residue in FlhA that couples export gate activation to substrate specificity switching in bacterial flagellar assembly.

Characterization of high affinity IgM and IgG monoclonal antibodies against norovirus variants GII.4 and GII.17

This study generates and characterizes monoclonal IgM and IgG antibodies against norovirus variants GII.4 and GII.17, revealing differences in binding mechanisms and affinity.

Ether phospholipids modulate somatosensory responses by tuning multiple receptor functions in Drosophila

This study shows that ether phospholipids modulate mechanosensory and thermosensory responses in Drosophila by affecting PIEZO and TRPA1 channels.

Key role of P230 in the hinge region on the IgG architecture and function

This study investigates the role of Pro230 in the hinge region of IgG by deleting it, revealing structural changes and functional effects on FcγRI-mediated activity.

Submolecular-scale Hairpin DNA Folding Dynamics Studied by High-Speed AFM with Optical Tweezers

This study combines optical tweezers with high-speed AFM to visualize DNA folding dynamics, revealing reversible structural changes and metastable intermediates.

A dual-domain chitinase mechanism enables marine bacteria tosense and localize sparse crystalline chitin in the ocean

This study identifies a dual-domain chitinase in marine bacteria that enhances chitin binding and retention, improving resource acquisition in the ocean.

Energy conversion mechanism revealed by ATP-free and ATP-dependent walking of myosin V motor

This study shows that myosin V can perform ATP-free walking by converting strain energy from actin binding into mechanical work, similar to ATP-dependent walking.

Structural basis for the interaction between the bacterial cell division proteins FtsZ and ZapA

This study reports the cryo-EM structure of the ZapA-FtsZ complex, revealing how ZapA interacts with FtsZ to regulate bacterial cell division.

A look beyond topography: transient phenomena of Escherichia coli cell division captured with high-speed in-line force mapping

This paper uses high-speed in-line force mapping to study dynamic mechanical properties of living E. coli during division, revealing transient phenomena like localized stiffening and membrane pore formation.

Structural analysis of S-ring composed of FliFG fusion proteins in marine Vibrio polar flagellar motors

The paper reports the near-atomic structure of the S-ring from Vibrio alginolyticus flagellar motor using cryo-EM, revealing structural differences compared to Salmonella.

Nanoscale visualization of Drosophila E-cadherin ectodomain fragments and their interactions using DNA origami nanoblocks

This paper uses DNA origami nanoblocks to visualize DE-cadherin ectodomain interactions and reveal a hinge-like structure involved in mechanical flexibility and adhesion-like processes.

Structure of the Human ATAD2 AAA+ Histone Chaperone Reveals Mechanism of Regulation and Inter-subunit Communication

The paper presents the cryo-EM structure of human ATAD2 in the ATP state and its complex with histone H3H4, revealing structural organization and a regulatory mechanism involving inter-subunit communication.

Key Nucleation Stages and Associated Molecular Determinants and Processes in pH-Induced Formation of Amyloid Beta Oligomers as Revealed by High-Speed AFM

This study uses high-speed AFM to visualize pH-induced AB42 peptide nucleation and identifies key molecular stages in oligomer formation.