Keir Cajal Neuman

Type II topoisomerase substrate geometry revealed through combined experiment and computation.

This study determines the 3D geometry of DNA crossings preferred for strand passage by topo II enzymes using single-molecule experiments and simulations.

Topoisomerase IIIα resolves inter- and intra-molecular intertwines during DNA replication

This study shows that TOP3 resolves both inter- and intra-molecular DNA intertwines during replication, promoting fork progression and daughter strand unlinking, especially when TOP2 is compromised.

Bacterial type II topoisomerases cleave DNA in a species-specific manner

The paper maps DNA cleavage sites of type II topoisomerases in three bacterial species, showing species-specific and drug-induced differences in cleavage specificity.

Highly sensitive mapping of in vitro type II topoisomerase DNA cleavage sites with SHAN-seq

This study develops SHAN-seq to map type II topoisomerase cleavage sites on DNA with high sensitivity, revealing hundreds of sites and enzyme-specific variations.

UPF1 ATPase autoinhibition and activation modulate RNA binding kinetics and NMD efficiency

This study investigates how UPF1's ATPase autoinhibition and activation affect RNA binding kinetics and NMD efficiency, revealing that UPF1-RNA interaction kinetics are critical for NMD.

UPF1 mutants with intact ATPase but deficient helicase activities promote efficient nonsense-mediated mRNA decay

The paper identifies UPF1 mutants with impaired helicase activity but intact ATPase activity that still support efficient nonsense-mediated mRNA decay in human cells.

Supercoiling DNA locates mismatches

This study demonstrates a method using magnetic tweezers to detect single base pair mismatches in DNA by observing plectoneme pinning under specific conditions.