Yuichiro Takagi

This study investigates how a specific mutation in the ASNS gene impairs enzyme function by disrupting conformational dynamics, using biochemical, cryo-EM, and molecular dynamics approaches.

The paper describes how ASX-173 inhibits asparagine synthetase, induces the integrated stress response, and reduces cell growth in HEK-293A cells.

The paper uses 3DVA and MD simulations to reveal that Arg-142 in ASNS acts as a gate controlling ammonia transport through an intramolecular tunnel.

The paper identifies molecular glue degraders that selectively degrade APP via CAPRIN1-dependent lysosomal pathway in neurons, reducing Aβ production in AD mouse models.

Positional scanning peptide arrays profile substrate sequence specificities across 303 human serine/threonine kinases, enabling computational kinase-substrate annotation and signaling pathway inference across the human phosphoproteome.

This paper reports the structure of Arabidopsis RDR2 and shows how it initiates transcription from RNA templates displaced from RNA-DNA hybrids.

The paper determines the X-ray crystal structure of human ASNS and builds a computational model of the enzyme-inhibitor complex to understand specificity.

The paper develops a method to estimate initial infectivity and optimize protein production using the baculovirus expression vector system.