Shigeyuki Shichino

Comprehensive and accelerated mapping of driver mutations through single-nucleotide random mutagenesis of target genes

This study introduces a genome editing method to rapidly generate and test driver mutations in target genes, identifying novel resistance mutations in lung cancer.

LAG-3 blockade reactivates the CD8+ T cell expansion program to re-expand contracted clones in the tumor

This study identifies a transcriptomic signature predicting CD8+ T cell clone expansion rates and shows LAG-3 blockade reactivates this signature to re-expand contracted clones in tumors.

Local niche-derived immunosuppressive CXCR2+ cells impair antiviral immunity

The paper identifies CXCR2+ MDSC-like cells in the olfactory bulb that suppress antiviral immunity, leading to worse outcomes after VSV infection.

TAS-Seq: a robust and sensitive amplification method for bead-based scRNA-seq

TAS-Seq improves scRNA-seq by enhancing gene detection and cell-cell interaction analysis in murine and human lung samples compared to existing methods.