António Pinto‐Duarte

Maternal Immune Activation Disrupts Epigenomic and Functional Maturation of Cortical Excitatory Neurons

This study shows that maternal immune activation disrupts DNA methylation and transcriptome of cortical excitatory neurons, delaying their maturation and affecting synapse-related genes.

Delivery of small interfering RNA and antisense oligonucleotides across the blood-brain barrier with monovalent transferrin receptor 1 binding VHH-Fc fusion proteins

This paper develops a VHH-Fc fusion protein to deliver siRNAs and ASOs across the BBB in rodents and NHPs, showing that binding affinity, DAR, and conjugation site affect delivery efficiency.

Single-cell DNA Methylome and 3D Multi-omic Atlas of the Adult Mouse Brain

The paper generates a single-cell DNA methylome and 3D multi-omic atlas of the adult mouse brain, identifying methylation patterns and regulatory networks across cell types and brain regions.

A comparative atlas of single-cell chromatin accessibility in the human brain

The paper constructs a single-cell chromatin accessibility atlas of the human brain, identifies cell-type-specific regulatory elements, and develops models to predict regulatory function of disease risk variants.

Laws for Glia Organization Conserved Across Mammals

The paper examines glia organization across mammalian species and finds that glia volume density is conserved within brain regions but varies between regions and scales with brain size.

Robust enhancer-gene regulation identified by single-cell transcriptomes and epigenomes

The paper identifies gene-CRE pairs in mouse brain cells by integrating single-cell transcriptomes and epigenomes, controlling for gene co-expression.

Brain-wide Correspondence Between Neuronal Epigenomics and Long-Distance Projections

The paper links single-cell epigenomes and projection patterns of 33,034 mouse neurons to identify transcriptomic and epigenomic properties of projection cell types.

Dnmt3a knockout in excitatory neurons impairs postnatal synapse maturation and is partly compensated by repressive histone modification H3K27me3

This study shows that Dnmt3a knockout in mouse excitatory neurons disrupts synapse maturation and working memory, with partial compensation by H3K27me3.

Evolution of cellular diversity in primary motor cortex of human, marmoset monkey, and mouse

The paper compares cellular diversity in the primary motor cortex across human, marmoset, and mouse using single-nucleus transcriptomics and epigenomics, finding conserved cell types and species-specific adaptations.

An Atlas of Gene Regulatory Elements in Adult Mouse Cerebrum

The paper identifies 491,818 candidate cis regulatory elements in adult mouse brain sub-types and links them to target genes and transcriptional regulators involved in various pathways.

An integrated transcriptomic and epigenomic atlas of mouse primary motor cortex cell types

The paper creates a comprehensive atlas of mouse primary motor cortex cell types using integrated transcriptomic and epigenomic data from over 600,000 samples.

DNA Methylation Atlas of the Mouse Brain at Single-Cell Resolution

The paper constructs a DNA methylation atlas of the mouse brain at single-cell resolution, revealing epigenetic diversity and spatial organization of neuronal subtypes.

Epigenomic Diversity of Cortical Projection Neurons in the Mouse Brain

The paper links epigenomic properties of cortical projection neurons to their anatomical and projection patterns using single-nucleus DNA methylation sequencing in mice.

Robust single-cell DNA methylome profiling with snmC-seq2

This paper introduces snmC-seq2, an improved single-cell DNA methylome profiling method that enhances read mapping, reduces artifacts, and increases throughput and coverage uniformity.