Sachin Kote

STAT3 dual role in sex-biased and differentiation-gated interferon gamma memory

The paper investigates the role of STAT3 in IFN-gamma memory in human macrophages, revealing its dual function in repression and recall, and identifies sex-biased and differentiation-dependent mechanisms.

Novel native serum peptidomics workflow enables the discovery of circulating subtype-specific peptide biomarkers in acute ischemic and haemorrhagic stroke

This paper introduces a novel serum peptidomics workflow that improves peptide yield, throughput, and recovery, identifying subtype-specific peptide biomarkers for stroke subtypes.

Benchmarking five extracellular vesicle proteomics workflows, including low-input Exo-insert and Exo-SP3, for deep mass spectrometry profiling

The paper benchmarks five proteomics workflows for sEV analysis, showing Exo-insert and Exo-SP3 can identify ~1100 proteins from low-input samples, with performance varying by sEV source.

Mature tumoroids recapitulate clinically relevant drug response through extended 3D culture in PDAC

This study shows that mature tumoroids, grown in 3D culture for ≥10 days, better reflect clinical drug responses in PDAC compared to short-term cultures by capturing microenvironmental effects and transporter-mediated resistance.

Universal toolset for mass spectrometric analysis of intracellular peptidome and small protein fraction

The paper describes methods for isolating and analyzing short intracellular polypeptides and small proteins, along with computational tools for data analysis.

The immunopeptidome from a genomic perspective: Establishing immune-relevant regions for cancer vaccine design

The paper develops COD-dipp to map MHC Class I antigens from cancer studies and identifies immune-relevant genomic regions for vaccine design.