Combi-PAINT improves multiplexing in super-resolution microscopy by using combinatorial DNA barcoding, resolving 41 targets with high accuracy in under 30 minutes.

bioRxiv · Jan 25, 2026 · PreprintBiophysics

Mitochondrial proteostatic stress disrupts mitoribosome biogenesis and translation

Hauke Holthusen, Victoria A. Trinkaus, Carina Fernandez Gonzalez, Itika Saha, Isabelle Pachmayr, Orsolya Kimbu Wade, Anja Deiser, Barbara Hummel, Patricia Yuste‐Checa, Kenneth Ehses, Roman Körner, Rubén Fernández‐Busnadiego, Ritwick Sawarkar, Ralf Jungmann, Mark S. Hipp, F. Ulrich Hartl

This study shows that proteostatic stress in mitochondria impairs mitoribosome biogenesis and translation, leading to respiratory deficiency and mitochondrial DNA loss.

bioRxiv · Sep 5, 2025 · PreprintCell Biology

Angstrom-resolution imaging of cell-surface glycans

Luciano A. Masullo, Karim Almahayni, Isabelle Pachmayr, Monique Honsa, Larissa Heinze, Sarah Fritsche, Heinrich Grabmayr, Ralf Jungmann, Leonhard Möckl

This study visualizes individual sugars within glycans on the cell surface using angstrom-resolution fluorescence microscopy combined with metabolic labeling.

Nature Nanotechnology · Jul 28, 2025Peer-reviewedBiophysics