Rubén Fernández‐Busnadiego

Molecular mechanisms of E-Syt-mediated stress resistance

This study identifies the molecular mechanisms by which E-Syt proteins mediate stress resistance through ER-PM MCS targeting and domain-specific functions.

Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress

The paper uses cryo-electron tomography to study structural changes in mitochondrial protein biogenesis under proteostatic stress, revealing remodeling of cristae and mHsp60 conformation.

Establishing synthetic ribbon-type active zones in a heterologous expression system

The paper reconstitutes synthetic ribbon-type active zones in HEK293 cells using Bassoon, RIBEYE, and CaV1.3 channels, showing structural and functional similarities to native ribbons.

The Myo2 adaptor Ldm1 and its receptor Ldo16 mediate actin-dependent lipid droplet motility

The paper identifies Ldm1 and Ldo16 as actin-dependent lipid droplet motility mediators in yeast, showing their roles in LD movement and mitochondrial transport.

Mitochondrial proteostatic stress disrupts mitoribosome biogenesis and translation

This study shows that proteostatic stress in mitochondria impairs mitoribosome biogenesis and translation, leading to respiratory deficiency and mitochondrial DNA loss.

SynapseNet: Deep Learning for Automatic Synapse Reconstruction

SynapseNet automatically segments synapses in electron micrographs using deep learning, enabling efficient analysis of synaptic structures.

Condensates of synaptic vesicles and synapsin are molecular beacons for actin sequestering and polymerization

The paper investigates how synapsin-SV condensates organize presynaptic actin through LLPS, revealing their role in SV clustering and actin polymerization in lamprey and mammalian synapses.

A correlative workflow streamlines synaptic imaging by cryo-electron tomography

The paper develops a correlative cryo-light/electron microscopy workflow to target and image synapses with cryo-ET, revealing excitatory synapses.

Temporal control of acute protein aggregate turnover by UBE3C and NRF1-dependent proteasomal pathways

This study identifies UBE3C and NRF1-dependent proteasomal pathways that regulate acute protein aggregate turnover in response to misfolded protein accumulation.

The molecular mechanism of on-demand sterol biosynthesis at organelle contact sites

This study identifies Yet3 as a protein that recruits sterol biosynthesis enzymes to ER contact sites, enabling on-demand sterol production and influencing organellar structure and function.

Gel-like inclusions of C-terminal fragments of TDP-43 sequester and inhibit proteasomes in neurons

This study identifies TDP-25 inclusions as sequestering proteasomes, leading to proteostasis impairment in ALS/FTD.

Estimation of Membrane Curvature for Cryo-Electron Tomography

The paper develops a method to estimate membrane curvature from cryo-ET data, showing its validity on benchmark surfaces and application to biological data.