Takashi Murayama

Structural basis for Ca2+-dependent allosteric modulation of cardiac ryanodine receptor by Ryanozole

This study defines the mechanism of Ryanozole's Ca2+-dependent modulation of RyR2 using cryo-EM, mutagenesis, and functional assays, revealing its structural basis for CPVT therapy.

A novel RyR2-selective stabilizer prevents stress-induced ventricular arrhythmias without impairing cardiac function

Ryanozole, a RyR2-selective stabilizer, prevents stress-induced arrhythmias without impairing cardiac function in CPVT mouse models.

Baculovirus-mediated gene transfer enables functional expression of PIEZO1 ion channel in isolated muscle satellite cells

This study develops a baculovirus-mediated method to express PIEZO1 in muscle satellite cells, restoring their proliferative capacity and enabling functional studies of large proteins.

Dantrolene inhibition of ryanodine receptor 1 carrying the severe malignant hyperthermia mutation Y522S visualized by cryo-EM

This study uses cryo-EM to visualize how dantrolene inhibits the Y522S RyR1 mutant, revealing its binding site and mechanism of action in preventing malignant hyperthermia.

Carvedilol suppresses ryanodine receptor-dependent Ca2+ bursts in human neurons bearing PSEN1 variants found in early onset Alzheimer's disease

The paper shows that PSEN1 variants in early-onset Alzheimer's disease cause abnormal Ca2+ bursts in neurons via RyR2, which can be suppressed by carvedilol.

Domain organization and conformational change of dynactin p150

This study uses electron microscopy to investigate the structure and conformational changes of the dynactin p150 subunit, revealing domain organization and a flexible sidearm with a coiled-coil domain.