Guido Marcucci

Resolving Immune Lineage and Cell-State Heterogeneity in Human PBMCs via Mass Spectrometry-Based Single-Cell Proteomics

The paper develops a pipeline for protein-level cell annotation in human PBMCs using mass spectrometry-based single-cell proteomics and validates it on unsorted samples.

Multiomic State-Transitions Reveal Post-Treatment Transcriptome Desynchronization in Acute Myeloid Leukemia

The paper uses a state-transition model to study transcriptome dynamics in AML, finding delayed miRNA responses and linking the Dlk1-Dio3 locus to treatment-induced desynchronization.

Longitudinal single cell RNA-sequencing reveals evolution of micro- and macro-states in chronic myeloid leukemia

The paper shows that clinically relevant leukemia phenotypes emerge at the macrostate level from single cell data, revealing how disease states aggregate from noisy single cell transcriptomes.

Prognostic and Therapeutic Implications of BRAF Mutations in Acute Myeloid Leukemia

This study identifies BRAF mutations in AML, showing poor outcomes and unique co-mutation patterns, suggesting potential targeted therapies.

Overcoming treatment resistance mediated by the bone marrow vascular niche in acute myeloid leukemia

This study develops a computational model of the AML bone marrow microenvironment and shows that combining TKIs with miR-126 inhibition can overcome treatment resistance by disrupting the protective vascular niche.

Targeted Irradiation and STAT3 Inhibition Reprogram the AML Microenvironment and Extend Survival: Toward Translational Immunoradiotherapy

This study combines targeted marrow irradiation with a STAT3 inhibitor to improve leukemia clearance and immune memory in AML models.