Agnieszka Gambus

Under or Over? Tracing Complex DNA Structures with High-Resolution Atomic Force Microscopy

This paper introduces a high-throughput AFM-based pipeline using deep learning to trace DNA topology, revealing new insights into DNA structures and their interactions.

RNA Polymerase II subunits overexpressions induce genome instability and deregulate transcription

The paper shows that overexpression of RNA Polymerase II subunits correlates with poor cancer outcomes and induces genome instability through subunit-specific transcriptional changes.

Profiling ubiquitin signaling with UBIMAX reveals DNA damage- and SCFβTRCP-dependent ubiquitylation of the actin-organizing protein Dbn1

The paper introduces UBIMAX, a method to profile ubiquitylation, and identifies Dbn1 as a novel DNA damage-induced ubiquitylation target regulated by SCFβTRCP.

A cell cycle-coordinated nuclear compartment for Polymerase II transcription encompasses the earliest gene expression before global genome activation

The paper identifies a nuclear transcription compartment that enables early gene expression before genome activation in metazoan embryos.

Mitotic replisome disassembly in vertebrates

The paper shows that mitotic replisome disassembly in vertebrates depends on TRAIP-mediated ubiquitylation of Mcm7 and p97/VCP activity, and is conserved across higher eukaryotes.