Stavros Stavrakis

CascadeMAP: Autonomous Closed-loop Optimization of Enzyme Cascades via Microfluidics, Machine Learning and Agentic AI

Unattended droplet-microfluidic Bayesian optimization of a glycerol detection cascade matched design of experiments (DoE) with one third of the conditions; a second cascade used DoE with Raman readout.

Uncovering Functional Distant Mutations by Ultra-High-Throughput Screening of Dehalogenases

This study uses ultrahigh-throughput screening to identify mutations in dehalogenases that modulate substrate access and binding, revealing distal functional effects on enzyme dynamics.

Structural mechanism governing radiationless energy transfer in Renilla bioluminescence

This study reports co-crystal structures of a bioluminescent energy-transfer complex in Renilla reniformis, revealing the spatial arrangement of donor-acceptor chromophores and their role in efficient radiationless energy transfer.

Droplet single-cell CRISPR screens identify regulators of T cell-mediated target-cell killing

The paper uses droplet single-cell CRISPR screening to identify regulators of T cell-mediated target-cell killing and finds that mTORC1 modulation enhances cytotoxic activity.

Conformational gating of product release sets the catalytic ceiling of a bioluminescent reporter

This study identifies conformational dynamics as a key determinant of luciferase catalytic efficiency and light-emission stability, revealing a trade-off between oxygen affinity and enzyme longevity.

In-vitro and in-vivo characterization of CRANAD-2 for multi-spectral optoacoustic tomography and fluorescence imaging of amyloid-beta deposits in Alzheimer mice

This paper characterizes CRANAD-2 for detecting amyloid-beta deposits in Alzheimer's mouse models using multi-spectral optoacoustic tomography and fluorescence imaging.