VitriFlex is an open-source robotic platform for cryo-EM grid preparation that enables customizable and high-resolution grid production through modular design and programmable control.

bioRxiv · Jul 24, 2025 · PreprintBiophysics

Molecular basis of the urate transporter URAT1 inhibition by gout drugs

Yang Suo, Justin G. Fedor, Han Zhang, Kalina Tsolova, Xiaoyu Shi, Kedar Sharma, Shweta Kumari, Mario Juan Borgnia, Peng Zhan, Wonpil Im, Seok‐Yong Lee

This study determines the cryo-EM structures of URAT1 with inhibitors and reveals their binding mechanisms and conformational changes.

Nature Communications · Jun 4, 2025Peer-reviewedBiophysics

Cryo-EM structures of engineered Shiga toxin-based immunogens capable of eliciting neutralizing antibodies with therapeutic potential against Hemolytic Uremic Syndrome

Alejandro Ezequiel Cristófalo, Arvind Sharma, María Laura Cerutti, Kedar Sharma, Roberto Melero, Romina Paola Pardo, Fernando Alberto Goldbaum, Mario Juan Borgnia, Vanesa Zylberman, Lisandro Horacio Otero

This paper presents cryo-EM structures of engineered Shiga toxin-based immunogens that stabilize pentamers and show interaction with neutralizing antibodies, supporting their use in a vaccine for STEC-HUS.

Protein Science · May 24, 2025Peer-reviewedImmunology

A humanized nanobody phage display library yields potent binders of SARS CoV-2 spike

Ying Fu, Juliana da Fonseca Rezende e Mello, Bryan D. Fleming, Alex Renn, Catherine Z. Chen, Xin Hu, Miao Xu, Kirill Gorshkov, Quinlin Hanson, Wei Zheng, Emily M. Lee, Lalith Perera, Robert M. Petrovich, Manisha Pradhan, Richard T. Eastman, , Anton M. Simeonov

This paper develops potent humanized nanobodies against SARS-CoV-2 spike protein, showing effectiveness against the Alpha variant and providing structural insights into their neutralization mechanism.

PLoS ONE · Aug 10, 2022Peer-reviewedSynthetic Biology