Bernhard Schmierer

ZEB1, a novel regulator of Junctional Adhesion Molecule A, impacts sensitivity of pancreatic cancer-associated fibroblasts to oncolytic reovirus

This study identifies ZEB1 as a regulator of JAM-A expression in pancreatic fibroblasts, showing that ZEB1 inhibition enhances reovirus sensitivity in these cells.

A genome-wide genetic screen identified targets for destabilizing the parasitophorous vacuole of Chlamydia trachomatis

The paper identifies CpoS as a key effector for maintaining Chlamydia trachomatis vacuole integrity by regulating sphingolipid supply and shows that disrupting this pathway can clear infection without harming host cells.

Rapid genome editing by CRISPR-Cas9-POLD3 fusion

The paper evaluates DNA repair protein-Cas9 fusions for improving CRISPR editing efficiency, finding that most require optimization for specific cell types and genomic loci.

Inhibition of p53 improves CRISPR/Cas-mediated precision genome editing

Cas9-induced double-strand breaks trigger p53/p21/RB1-dependent G1 arrest in RPE-1 cells; transient p53 inhibition via MDM2 markedly raises homologous recombination precision editing efficiency.

CRISPR/Cas9 screening using unique molecular identifiers

This paper introduces RSLs as UMIs to improve CRISPR/Cas9 screening reproducibility by enhancing precision and reducing required cells and sequencing reads.