Tamir Gonen

Characterization of energy filtering slit widths for MicroED data collection

This study evaluates energy filter slit widths in MicroED to improve signal-to-noise ratio and data quality for macromolecular structure determination.

Inhibition of the Lysosomal Amino Acid Sensor SLC38A9 by the Membrane Microprotein SPAR

This study identifies SPAR, a microprotein, as an inhibitor of SLC38A9, a lysosomal amino acid transporter, revealing its role in mTORC1 pathway regulation by blocking arginine transport and Rag GTPase recruitment.

pH-dependent regulation in SLC38A9

This study shows that SLC38A9, a lysosomal amino acid transporter, has pH-dependent arginine uptake regulated by the histidine residue His544, which influences transport through protonation/deprotonation rather than direct substrate binding.

Discovery of oxyacanthine dihydrochloride monohydrate polymorphs from obfuscated samples by MicroED

This study identifies two polymorphic structures of oxyacanthine dihydrochloride monohydrate using MicroED, revealing conformational differences and thermodynamic preferences.

Biophysical Basis of Paracellular Barrier Modulation by a Pan-Claudin-Binding Molecule

The paper develops a synthetic antibody fragment that binds to 10 claudin subtypes, opening tight junctions and modulating paracellular permeability.

High-throughput MicroED for probing ion channel dynamics

This study uses high-throughput MicroED to analyze structural dynamics of NaK and NaK2CNG ion channels, revealing sodium binding and dynamic selectivity filter behavior.

Recovering high-resolution information using energy filtering in MicroED

This study shows energy filtering in MicroED improves data quality by reducing noise, sharpening Bragg peaks, and recovering high-resolution information despite increased radiation damage.

Ligand Screening and Discovery using Cocktail Soakingand Automated MicroED

This study develops a workflow combining cocktail soaking with automated microED to enable rapid ligand screening and structure determination from microcrystals, validated with known and novel ligand interactions.

Structure of the lens MP20 mediated adhesive junction

This study determines the structure of MP20, a lens membrane protein, revealing its tetrameric assembly and adhesive function in forming thin junctions essential for lens transparency.

Energy filtering enables macromolecular MicroED data at sub-atomic resolution

This study shows that energy filtering improves MicroED data quality, enabling sub-atomic resolution and better structure modeling of proteinase K crystals.

MicroED Structures of Fluticasone Furoate and Fluticasone Propionate Provide New Insights to Their Function

This study uses MicroED and DFT to determine the 3D structures of fluticasone furoate and propionate in different states, revealing conformational changes and their functional implications.

An Updated Structure of Oxybutynin Hydrochloride

This study determines the 3D structure of oxybutynin hydrochloride using MicroED and reveals insights into its binding mechanism with the M3 muscarinic receptor.

Near-Atomic Cryo-EM Imaging of a Small Protein Displayed on a Designed Scaffolding System

The paper demonstrates that small proteins can be visualized at near-atomic resolution using cryo-EM when attached to a symmetric protein scaffold.

A method to minimize condenser lens-induced hysteresis effects in a JEOL JEM-3200FSC microscope to enable stable cryoEM low-dose operations

The paper introduces a method to reduce magnetic hysteresis in a JEOL JEM-3200FSC microscope, enabling stable low-dose cryoEM operations by independently ramping condenser lenses before data collection.

MicroED Structures from Micrometer Thick Protein Crystals

This study shows that MicroED can determine atomic resolution protein structures from crystals up to one micrometer thick, much thicker than previously thought possible.