Nicholas C.K. Valerie

A modular lentiviral system for multiplexed gene perturbation and functional analysis suggests interdependence of hormone receptors in breast cancer growth in vivo

The paper introduces a modular lentiviral system for multiplexed gene perturbation and functional analysis, showing that ER+ breast cancer cells rely on AR, ER, and PR for in vivo growth.

NUDT5 regulates the global efficacy of nucleoside analog drugs by coordinating purine synthesis and PRPP allocation

This study shows NUDT5 regulates the effectiveness of nucleoside analog drugs by coordinating purine synthesis and PRPP allocation through its interaction with PPAT.

A cell-based degrader assessment platform facilitates discovery of functional NUDT5 PROTACs

This paper introduces a cell-based platform to assess PROTACs targeting NUDT5, identifying a VHL-dependent degrader despite poor in vitro binding and showing CRBN-dependent degraders are ineffective.

Uridine cytidine kinases dictate the therapeutic response of molnupiravir via its bioactivation

This study identifies uridine cytidine kinases (UCKs) as key enzymes that phosphorylate molnupiravir's active metabolite, influencing its antiviral efficacy and selectivity against SARS-CoV-2.

Duplexed CeTEAM drug biosensors reveal determinants of PARP inhibitor selectivity

This study uses CeTEAM biosensors to profile PARP inhibitor selectivity at the single-cell level, revealing PARP1 and PARP2 selectivity patterns and the role of HPF1 in drug binding.