Sean P. Palecek

This study identifies molecular pathways and regulatory programs associated with hPSC-CM maturation through multi-omic profiling during long-term culture.

The paper demonstrates a method to regulate the Wnt pathway in human pluripotent stem cells by linking a developmental promoter to CRISPR interference, showing reduced CTNNB1 expression and attenuated Wnt gene induction.

The paper identifies transcription factors that induce BBB properties in human pluripotent stem cell-derived endothelial cells through forward programming.

This paper shows that Human Plasma-Like Medium (HPLM) improves the maturation of human pluripotent stem cell-derived cardiomyocytes compared to conventional media.

This study develops hypoimmunogenic hPSC-derived cardiac organoids that evade immune rejection and repair damaged hearts in animal models.

This study shows that SNRK in atrial cardiomyocytes reduces TGFbeta1 levels, preventing fibrosis and heart failure.

This study shows that severe traumatic brain injury disrupts cerebral blood flow and leads to loss of ciliary protein ARL13B in endothelial cells.

This paper develops a high-throughput BTB assembloid model to study glioblastoma-neurovascular interactions and therapeutic responses, finding that BTB disruption correlates with increased tumor invasiveness and drug resistance.

The paper shows neuronal activity increases brain endothelial cholesterol synthesis and uptake, which inhibits arteriole dilation and acts as a negative feedback mechanism for neurovascular coupling.

The paper uses top-down proteomics to analyze MLC-1 and -2 isoform expression in human heart chambers, revealing chamber-specific patterns and a novel PTM.

This study shows that fibronectin promotes cardiac differentiation of human pluripotent stem cells, while laminins inhibit it, and identifies fibronectin's role in early differentiation through integrin signaling.

This study shows that activating Wnt signaling in human pluripotent stem cell-derived naive endothelial progenitors promotes the acquisition of blood-brain barrier properties.

The paper develops a multi-omics method to analyze metabolome and proteome of hPSC-CMs, identifying overrepresented pathways in protein synthesis and cardiac function.

This study derives and compares mesodermal and ectomesodermal chondrocytes from hiPSCs, showing that ectomesodermal-derived chondrocytes better resemble native chondrocytes and have improved cartilage repair capabilities.

The paper generates human pluripotent stem cell-derived brain pericyte-like cells that induce blood-brain barrier properties in endothelial cells and form an isogenic NVU model.

This paper develops a BBB chip that maintains high barrier function for a week, enabling better drug and antibody testing.