David S. Roberts

Structural Heterogeneity of Proteoform-Ligand Complexes in AMP-Activated Protein Kinase Uncovered by Integrated Top-Down Mass Spectrometry

This study uses integrated top-down mass spectrometry to reveal structural heterogeneity in AMPK proteoform-ligand complexes, uncovering new insights into kinase regulation.

Rational design of a foldon-derived heterotrimer guided by quantitative native mass spectrometry

This study designs a stable heterotrimeric protein complex using rational design and native mass spectrometry, validated by X-ray crystallography.

Directed Evolution of Genetically Encoded LYTACs for Cell-Mediated Delivery

The paper describes the development of genetically encodable LYTACs (GELYTACs) for targeted protein degradation using engineered T-cells, showing efficacy in secreting and delivering the therapeutics.

A Genome-Wide CRISPR Screen Identifies Sortilin as the Receptor Responsible for Galectin-1 Lysosomal Trafficking

The paper identifies sortilin as the receptor mediating galectin-1 lysosomal trafficking through a CRISPR screen and demonstrates its use for siRNA delivery.

Top-down Proteomics of Myosin Light Chain Isoforms Define Chamber-Specific Expression in the Human Heart

The paper uses top-down proteomics to analyze MLC-1 and -2 isoform expression in human heart chambers, revealing chamber-specific patterns and a novel PTM.

MASH Native: A Unified Solution for Native Top-Down Proteomics Data Processing

MASH Native provides a unified software solution for processing native top-down proteomics data, offering database searching and analysis tools in a user-friendly interface.

Bromodomain-containing Protein 4 Regulates Innate Inflammation in Airway Epithelial Cells via Modulation of Alternative Splicing

This study shows BRD4 regulates alternative splicing of immune-related genes in airway epithelial cells during viral infection, affecting innate inflammation and protein processing.

A Multi-omics Method Enabled by Sequential Metabolomics and Proteomics for Human Pluripotent Stem Cell-derived Cardiomyocytes

The paper develops a multi-omics method to analyze metabolome and proteome of hPSC-CMs, identifying overrepresented pathways in protein synthesis and cardiac function.

Ultrafast and Reproducible Proteomics from Small Amounts of Heart Tissue Enabled by Azo and timsTOF Pro

The paper introduces an ultrafast proteomics method using Azo and timsTOF Pro to enable high-throughput, reproducible protein identification and quantification from small amounts of human heart tissue.