This study modifies FcεR1γ with a 4-1BB co-stimulatory motif to enhance NK cell function against HLA-E+ cells, improving cytotoxicity in suppression-prone conditions.

The Journal of Immunology · Nov 2, 2025Peer-reviewedImmunology

Site-specific genome engineering of primary human natural killer cells for programmable anti-tumor function

Vincent Allain, Allison Grace Rothrock, Pierre-Louis Bernard, William A. Nyberg, Alexis Talbot, Joseph J. Muldoon, Jing-Yi Chung, Angela To, Christopher R. Chang, Gabriella R. Kimmerly, Chang Liu, Tasha Tsao, Yasaman Mortazavi, Jin Seo, Zhongmei Li, , Justin Eyquem

This paper develops a genome engineering workflow for primary human NK cells using CRISPR/Cas9 and AAV6 to integrate and disrupt genes, enhancing anti-tumor function through targeted CAR expression.

bioRxiv · Oct 5, 2025 · PreprintImmunology

Spleen Tyrosine Kinase (SYK) negatively regulates ITAM-mediated human NK cell signaling and CD19-CAR NK cell efficacy

Alberto Jovani Millan, Vincent Allain, Indrani Nayak, Jeremy B. Libang, Lilian M Quijada-Madrid, Janice S Arakawa-Hoyt, Gabriella Ureno, Allison Rothrock, Avishai Shemesh, Oscar A. Aguilar, Justin Eyquem, Jayajit Das, Lewis L. Lanier

This study shows that SYK negatively regulates NK cell signaling and that its deletion enhances CD19-CAR NK cell efficacy in cancer immunotherapy.

The Journal of Immunology · Mar 1, 2025Peer-reviewedImmunology

CITE-seq analysis reveals human cytomegalovirus and diabetes-associated adaptive NK cell alterations in cardiovascular disease.

Sujit Silas Armstrong Suthahar, Daniel G. Chen, Sunil Kumar, James R. Heath, Matthew J. Feinstein, John R. Greenland, Daniel R. Calabrese, Lewis L. Lanier, Klaus F. Ley, Avishai Shemesh

The paper uses CITE-seq to analyze NK cell alterations in CAD patients, finding SPON2/IFNG ratio and adaptive NK cell signatures correlate with disease severity and inflammation.

International Journal of Molecular Sciences · Feb 6, 2025Peer-reviewedImmunology