Advanced biosensing and bioanalysis techniques

This paper introduces a method to detect multiple molecular classes using nanopore sequencing by encoding targets into periodic DNA barcodes, achieving high specificity and error tolerance.

This paper introduces a DNA origami nanomechanical amplifier that translates molecular conformational changes into measurable mechanical displacements, enabling single-molecule analysis of binding events without labeling or fluorescence.

This paper develops a CRISPR/Cas12a-based system for sensitive and multiplexed detection of EV-derived microRNAs using DNAzyme and machine learning for cancer classification.

This study investigates gene-length DNA monolayers on gold electrodes for cell-free protein expression, showing tunable surface density and partial functional retention under certain conditions.

OrthoSeq designs DNA sequence-pair libraries with thermodynamic orthogonality, outperforming sequential methods in library size under constraints.

This paper introduces an automated design method for stiffness-tunable DNA origami nanostructures that can be manufactured into functional metamaterials.

This paper introduces molecular pendulum electrochemical sensors with active-reset mechanisms for real-time, ultrasensitive protein detection in biofluids.

The paper identifies DNA-DNA kissing complexes and develops an adenosine-triggered aptaswitch using them for controlled nanostructure assembly.

This paper demonstrates the co-assembly of DNA nanotubes and condensates in synthetic compartments, enabling programmable structures that mimic cellular organization.

This paper presents a method to achieve programmable and dynamic DNA localisation at synthetic cell membranes using RNA linkers and enzymatic degradation.

AptBacterialDB is a manually curated database of antibacterial aptamers with detailed annotations and integration tools to support research and development.

AptCancerDB curates experimentally validated anticancer aptamers, providing a resource for discovery and development of diagnostics and therapeutics.

This paper demonstrates that transcription-induced coacervation accelerates and enhances cell-free biosensing by mimicking intracellular phase separation, improving sensitivity and reducing detection time.

This paper develops an impedimetric aptasensor using DNA tetrahedral nanostructures for ultrasensitive IL-6 detection with a limit of detection of 55 ag/mL.

This paper introduces a computational pipeline using DNA k-mer imprints to agnostically classify and distinguish materials based on their sequence patterns.

This paper introduces an enzyme-free DNA strand displacement network that uses DNA templates to assemble non-covalent DNA multimers under isothermal conditions, achieving far-from-equilibrium product formation.

This paper develops DNA-RNA artificial cells with RNA organelles that enable spatially encoded functions and signal processing through aptamer-based protein recruitment and environmental responsiveness.

This paper develops an EpCAM-targeted L-DNA nanostructure for targeted doxorubicin delivery in TNBC, showing improved stability and reduced cytotoxicity in normal cells.

This study observes sub-microsecond dynamics of single DNA molecules using graphene-based fluorescence measurements and links findings to structural and environmental factors.

The paper demonstrates a DNA detection method using Mimiviral polymerase X (mvPolX) that is sequence-specific and sensitive, capable of detecting as few as 10 copies of target DNA in 15 minutes.

FINDER enables attomolar biomarker detection by converting kinetic fingerprinting into area-scalable sensitivity without amplification.

This paper uses Bayesian optimisation and graph-based rheology to model DNA materials by linking sequence design to material properties.

The paper demonstrates DNA condensate-based organelles that enable spatially regulated gene expression and protein targeting in synthetic cells, showing higher protein yield compared to standard PURE expression.

This paper develops an electrochemical aptasensor for ultrasensitive detection of IL-6 in clinical biofluids, showing high sensitivity and biocompatibility for septic cardiomyopathy diagnostics.

This paper develops a charge-amplified FET biosensor for detecting small-molecule biomarkers with picomolar sensitivity in complex biological samples.

This paper introduces a method to enhance DNA nanoswitch biosensor sensitivity by recycling targets through enzymatic ligation and cyclic annealing, enabling signal amplification without target amplification.

This paper introduces a method to encapsulate gold nanoparticles within DNA origami tubes to improve barcode stability and functionality for multiplexed biomolecular detection.

The paper designs DNA aptamers for Lyme disease detection by combining in silico selection using a Restricted Boltzmann Machine with experimental validation of selected sequences.

This study evaluates how physicochemical factors affect the stability of DNA tetrahedral nanostructures in vitro.